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Abstract：
The collection of T cell receptors (TCRs) generated by somatic recombination is large but unknown. We generate large TCR repertoire datasets as a resource to facilitate detailed studies of the role of TCR clonotypes and repertoires in health and disease. We estimate the size of individual human recombined and expressed TCRs by sequence analysis and determine the extent of sharing between individual repertoires. Our experiments reveal that each blood sample contains between 5 million and 21 million TCR clonotypes. Three individuals share 8% of TCRβ- or 11% of TCRα-chain clonotypes. Sorting by T cell phenotypes in four individuals shows that 5% of naive CD4+ and 3.5% of naive CD8+ subsets share their TCRβ clonotypes, whereas memory CD4+ and CD8+ subsets share 2.3% and 0.4% of their clonotypes, respectively. We identify the sequences of these shared TCR clonotypes that are of interest for studies of human T cell biology.
摘要：
通过体细胞重组产生的T细胞受体(TCR)的集合很大但未知。我们生成大型TCR库数据集作为资源，以促进对TCR克隆型和库在健康和疾病中的作用进行详细研究。我们通过序列分析估计单个人重组和表达的TCR的大小，并确定单个谱系之间的共享程度。我们的实验表明，每个血液样本包含500万至2100万种TCR克隆型。三个个体共享8%的TCRβ-或11%的TCRα-链克隆型。通过四个个体的T细胞表型排序显示，5％的幼稚CD4+和3.5％的幼稚CD8+亚群共享其TCRβ克隆型，而记忆CD4+和CD8+亚群共享其克隆型的2.3%和0.4%，分别。我们鉴定了这些共享TCR克隆型的序列，这些序列对人类T细胞生物学的研究感兴趣。
