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Abstract：
Wheat stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), is a fungus that causes the devastating fungalwheat stem rust disease in wheat production. Rapid identification of the physiological races of Pgt are very importance for the prevention of wheat stem rust. In this paper we developed a molecular method to identify the most prevalent race of Pgt, as a supplement for traditionally used host-specific methods. Amplified fragment length polymorphism (AFLP) was employed as a means of analyzing DNA polymorphisms in six common physiological races of Pgt in China and Ug99. In total, 64 pairs of primers were used for AFLP screening of race-specific molecular markers. One primer pair-namely, E7/M7 (5\'-GACTGCGTACCAATTCG G-3\'/5\'-GATGAGTCCTGAGTAACGG-3\')-yielded a unique band for the race 34MKG that was purified and cloned into the pGEM-T vector for sequencing. We then designed a new primer pairs (sequence-characterized amplified region marker) to amplify the 171-bp fragment and confirmed that the marker was highly specific for 34MKG. These results provide a new tool for monitoring different races of Pgt for improved control of wheat stem rust in China.
摘要：
小麦茎锈病，由普契氏菌引起。小麦（Pgt），是一种真菌，在小麦生产中引起毁灭性的冈麦茎锈病。快速鉴定Pgt的生理小种对于预防小麦茎锈病非常重要。在本文中，我们开发了一种分子方法来鉴定Pgt最普遍的种族，作为传统使用的宿主特异性方法的补充。扩增片段长度多态性（AFLP）被用作分析中国和Ug99Pgt六个常见生理种族的DNA多态性的手段。总的来说,64对引物用于AFLP筛选种族特异性分子标记。一对引物-即，E7/M7（5'-GACTGCGTACCAATTCGG-3\'/5'-GATGAGTCCTGAGTAACGG-3\'）产生了种族34MKG的独特条带，将其纯化并克隆到pGEM-T载体中进行测序。然后，我们设计了一种新的引物对（序列表征的扩增区标记）来扩增171bp的片段，并证实该标记对34MKG具有高度特异性。这些结果为监测不同品种的Pgt提供了新的工具，以改善中国小麦茎锈病的防治。
