Clubroot,由油菜疟原虫引起的,是全球范围内至关重要的油菜病害。有关十字花菜种群毒力的信息对于使用适当的抗根茎品种进行疾病控制至关重要。2016-2020年,在捷克共和国(CZ)收集了84株油菜分离株,德国(DE),波兰(PL),瑞典(SW)。使用17个芸苔属宿主指定了致病型,包括欧洲俱乐部差异(ECD),索美套装,和抗夜蛾油菜简历。孟德尔.根据ECD设置,毒力分析将分离株分为42种致病型。最常见的病理类型是16/31/31(在DE,PL,和SW)和16/06/12(CZ,DE,和PL)。根据Somé集发现了六种病理类型,每个国家包括1-4种病理类型。P1在DE中最普遍,PL,和SW,虽然P3在CZ中很丰富,DE,和PL。目前的研究提供了明确的证据,表明与以前的研究相比,在油菜种群中毒力增加。几种分离株克服了cv的抗性。孟德尔和芸苔属基因型ECD01至ECD04。考虑到所有调查的样本,在轮作中,根茎发病率与油菜频率之间存在显著的负相关,至于根肿病的发生率和土壤pH值。
Clubroot, caused by Plasmodiophora brassicae, is a crucial oilseed rape disease worldwide. Information on the virulence of P. brassicae populations is essential to apply disease control with proper clubroot-resistant cultivars. In 2016-2020, 84 isolates of P. brassicae were collected in the Czech Republic (CZ), Germany (DE), Poland (PL), and Sweden (SW). Pathotypes were designated using 17 Brassica hosts, including the European Clubroot Differentials (ECD), Somé set, and clubroot-resistant oilseed rape cv. Mendel. According to the ECD set, virulence analyses differentiated the isolates into 42 pathotypes. The most common pathotypes were 16/31/31 (in DE, PL, and SW) and 16/06/12 (in CZ, DE, and PL). Six pathotypes were found according to the Somé set, including 1-4 pathotypes per country. P1 was most prevalent in DE, PL, and SW, while P3 was abundant in CZ, DE, and PL. The current study provides clear evidence for a shift towards increased virulence in P. brassicae populations compared to previous studies. Several isolates overcame the resistance of cv. Mendel and of Brassica rapa genotypes ECD 01 to ECD 04. Considering all investigated samples, significant negative correlations were found between clubroot incidence and the frequency of oilseed rape in crop rotation, as for clubroot incidence and soil pH.