关键词: CRISPR-Cas Chromosomal integration Homology-mediated end joining Industrial biotechnology Site-specific recombination Synthetic biology
Mesh : Actinobacteria / genetics Chromosomal Instability Chromosomes Cloning, Molecular / methods Clustered Regularly Interspaced Short Palindromic Repeats DNA End-Joining Repair Escherichia coli / genetics metabolism Gene Dosage Genes Genome, Microbial Homologous Recombination Industrial Microbiology / methods Integrases / genetics metabolism Microorganisms, Genetically-Modified / genetics Multigene Family Synthetic Biology / methods Yeasts / genetics metabolism
来 源:
DOI:10.1016/j.biotechadv.2019.04.002
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