工程化微生物作为肿瘤治疗中的独特治疗平台已经引起了极大的兴趣。与传统的癌症治疗策略相比,工程微生物为基础的系统提供了各种独特的优势,例如靶向肿瘤的内在能力,它们固有的免疫原性,抗肿瘤药物的原位生产,和多种协同功能来对抗肿瘤。在这里,我们彻底审查了设计,准备,以及工程微生物在晚期肿瘤治疗中的应用。这篇综述提出了基于一系列代表性工程微生物的创新肿瘤治疗策略的全面调查,包括细菌,病毒,微藻,和真菌。具体来说,它提供了对设计原则的广泛分析,工程战略,和肿瘤治疗机制,以及不同工程微生物系统的优点和局限性。最后,我们讨论了该领域当前的挑战和未来的研究前景,这可以为利用工程微生物设计创新的肿瘤治疗范式提供新的思路,并促进其临床应用。本文受版权保护。保留所有权利。
Engineered microorganisms have attracted significant interest as a unique therapeutic platform in tumor treatment. Compared with conventional cancer treatment strategies, engineering microorganism-based systems provide various distinct advantages, such as the intrinsic capability in targeting tumors, their inherent immunogenicity, in situ production of antitumor agents, and multiple synergistic functions to fight against tumors. Herein, the design, preparation, and application of the engineered microorganisms for advanced tumor therapy are thoroughly reviewed. This review presents a comprehensive survey of innovative tumor therapeutic strategies based on a series of representative engineered microorganisms, including bacteria, viruses, microalgae, and fungi. Specifically, it offers extensive analyses of the design principles, engineering strategies, and tumor therapeutic mechanisms, as well as the advantages and limitations of different engineered microorganism-based systems. Finally, the current challenges and future research prospects in this field, which can inspire new ideas for the design of creative tumor therapy paradigms utilizing engineered microorganisms and facilitate their clinical applications, are discussed.