目的:细菌纤维素(BC)已显示出较高的创伤和烧伤治疗能力,提供潮湿的环境。海藻酸钙可以与BC结合以产生有助于伤口清创并有助于适当伤口愈合的凝胶。这项研究旨在表征和评估细菌纤维素/藻酸盐凝胶在大鼠皮肤烧伤中的用途。
方法:比较了纤维素和纤维素/藻酸盐凝胶的液体吸收能力,水分,粘度,和潜在的细胞毒性。2度烧伤是使用铝金属板(2.0cm)在120ºC下在大鼠背部进行20秒。将动物分为非治疗,CMC(羧甲基纤维素),纤维素(CMC与细菌纤维素),和纤维素/藻酸盐(具有细菌纤维素和藻酸盐的CMC)。动物接受3次/周的局部治疗。生化(MPO,NAG和氧化应激),14日进行组织形态学和免疫组织化学测定(IL-1βIL-10和VEGF),21日,28日,第35天
结果:与纤维素凝胶相比,纤维素/海藻酸盐凝胶显示出更高的吸收能力和粘度,没有细胞毒性作用。纤维素/藻酸盐呈现较低的MPO值,IL-10百分比更高,氧化应激谱更大且平衡。
结论:使用纤维素/藻酸盐凝胶可减少中性粒细胞和巨噬细胞的活化,并显示出更大的抗炎反应,这有助于治愈慢性伤口和烧伤。
OBJECTIVE: Bacterial cellulose (BC) has shown high capacity for the treatment of wounds and burns, providing a moisty environment. Calcium alginate can be associated with BC to create gels that aid in wound debridement and contribute to appropriate wound healing. This study is aimed at characterizing and evaluating the use of bacterial cellulose/alginate gel in skin burns in rats.
METHODS: Cellulose and cellulose/alginate gels were compared regarding the capacity of liquid absorption, moisture, viscosity, and potential cytotoxicity. The 2nd degree burns were produced using an aluminum metal plate (2.0cm) at 120ºC for 20s on the back of rats. The animals were divided into non-treated, CMC(Carboxymethylcellulose), Cellulose(CMC with bacterial cellulose), and Cellulose/alginate(CMC with bacterial cellulose and alginate). The animals received topical treatment 3 times/week. Biochemical (MPO, NAG and oxidative stress), histomorphometry and immunohistochemical assays (IL-1β IL-10 and VEGF) were conducted on the 14th, 21st, 28th, and 35th days.
RESULTS: Cellulose/Alginate gel showed higher absorption capacity and viscosity compared to Cellulose gel, with no cytotoxic effects. Cellulose/alginate presented lower MPO values, a higher percentage of IL-10, with greater and balanced oxidative stress profile.
CONCLUSIONS: The use of cellulose/alginate gel reduced neutrophils and macrophage activation and showed greater anti-inflammatory response, which can contribute to healing chronic wounds and burns.