Abstract：
Safe and effective anti-rabies vaccines are intensely sought worldwide. DNA vaccines have already shown their efficacy and safety and have occupied a special place in the field. Two prototype anti-rabies DNA vaccines were compared for the potential to induce virus-specific antibody production. One vector contained a codon-optimized gene with a territory-adapted consensus sequence of the rabies virus glycoprotein. The other one expressed the same glycoprotein in fusion with a c-CD63 lysosome targeting motif at the C terminus. ELISA of serum samples from immunized mice showed that the c-CD63 variant induced more efficient antibody production and shifted the IgG2a/IgG1 ratio towards the Th2-type immune response. The results gave grounds to believe that the approach successfully applied to the rabies glycoprotein may help to develop new-generation anti-rabies vaccines.
摘要：
安全和有效的抗狂犬病疫苗在世界范围内被强烈地寻求。DNA疫苗已经显示出它们的功效和安全性，并在该领域占据了特殊的位置。比较了两种原型抗狂犬病DNA疫苗诱导病毒特异性抗体产生的潜力。一种载体包含密码子优化的基因，该基因具有狂犬病病毒糖蛋白的区域适应的共有序列。另一个在C末端与c-CD63溶酶体靶向基序融合表达相同的糖蛋白。来自免疫小鼠的血清样品的ELISA显示,c-CD63变体诱导更有效的抗体产生并使IgG2a/IgG1比率向Th2型免疫应答转变。结果使人们有理由相信，该方法成功应用于狂犬病糖蛋白可能有助于开发新一代抗狂犬病疫苗。
