{Reference Type}: Journal Article
{Title}: [Rabies Virus Glycoprotein with a Consensus Amino Acid Sequence and a Lysosome Targeting Signal Causes Effective Production of Antibodies in DNA-Immunized Mice].
{Author}: Starodubova ES;Kuzmenko YV;Pankova EO;Latanova AA;Preobrazhenskaya OV;Karpov VL;
{Journal}: Mol Biol (Mosk)
{Volume}: 52
{Issue}: 2
{Year}: Mar-Apr 2018
暂无{DOI}: 10.7868/S0026898418020155
{Abstract}: Safe and effective anti-rabies vaccines are intensely sought worldwide. DNA vaccines have already shown their efficacy and safety and have occupied a special place in the field. Two prototype anti-rabies DNA vaccines were compared for the potential to induce virus-specific antibody production. One vector contained a codon-optimized gene with a territory-adapted consensus sequence of the rabies virus glycoprotein. The other one expressed the same glycoprotein in fusion with a c-CD63 lysosome targeting motif at the C terminus. ELISA of serum samples from immunized mice showed that the c-CD63 variant induced more efficient antibody production and shifted the IgG2a/IgG1 ratio towards the Th2-type immune response. The results gave grounds to believe that the approach successfully applied to the rabies glycoprotein may help to develop new-generation anti-rabies vaccines.