泼妇是食虫动物,作为一系列人畜共患病毒的天然储库,包括最近于2018年在中国发现的狼牙网病毒(LayV)。重要的是要了解与泼妇有关的病毒,病毒多样性,和新的病毒。在目前的研究中,我们对从中国东部沿海捕获的398只shrew的肺样本进行了高通量测序,并具有6种常见的shrew种(Anourosorexsquamipes,Crociduralasiura,龙骨山,谷草,Sorexcaecutiens,和Suncusmurinus)。我们的分析揭示了包括54种已知病毒和72种新病毒的许多与泼妇相关的病毒,这些病毒显著增强了我们对哺乳动物病毒的理解。值得注意的是,34种鉴定的病毒具有潜在的溢出风险,6种是人类致病病毒:LayV,甲型流感病毒(H5N6)轮状病毒A,狂犬病病毒,禽副粘病毒1和大鼠戊型肝炎病毒。此外,在中国发现了10种以前未报告的病毒,其中六个具有溢出风险潜力。此外,所有54种已知病毒和12种新病毒都具有跨越物种界限的能力.我们的数据强调了与shrew相关的病毒的多样性,并为进一步研究追踪和预测源于shrew的新出现的传染病奠定了基础。
Shrews being insectivores, serve as natural reservoirs for a wide array of zoonotic viruses, including the recently discovered Langya henipavirus (LayV) in China in 2018. It is crucial to understand the shrew-associated virome, viral diversity, and new viruses. In the current study, we conducted high-throughput sequencing on lung samples obtained from 398 shrews captured along the eastern coast of China, and characterized the high-depth virome of 6 common shrew species (Anourosorex squamipes, Crocidura lasiura, Crocidura shantungensis, Crocidura tanakae, Sorex caecutiens, and Suncus murinus). Our analysis revealed numerous shrew-associated viruses comprising 54 known viruses and 72 new viruses that significantly enhance our understanding of mammalian viruses. Notably, 34 identified viruses possess spillover-risk potential and six were human pathogenic viruses: LayV, influenza A virus (H5N6), rotavirus A, rabies virus, avian paramyxovirus 1, and rat hepatitis E virus. Moreover, ten previously unreported viruses in China were discovered, six among them have spillover-risk potential. Additionally, all 54 known viruses and 12 new viruses had the ability to cross species boundaries. Our data underscore the diversity of shrew-associated viruses and provide a foundation for further studies into tracing and predicting emerging infectious diseases originated from shrews.