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Abstract：
We investigated the presence and epidemiological relatedness of Salmonella isolates from a wastewater treatment plant (WWTP) in Brno, Czech Republic and from nestlings of black-headed gulls (Chroicocephalus ridibundus) at the Nove Mlyny waterworks, situated 35 km downstream from the WWTP. During 2012, we collected 37 wastewater samples and 284 gull cloacal swabs. From wastewater samples, we obtained 89 Salmonella isolates belonging to 19 serotypes. At least one resistant strain was contained in 89% of those samples. Ten different serotypes of Salmonella were detected in 38 young gulls, among which 14 (37%) were resistant to antimicrobials. Wastewater isolates were mostly resistant to sulphonamides and tetracycline, gull isolates to tetracycline and ampicillin. We detected the occurrence of blaTEM-1,tet(A), tet(B), tet(G), sul1, sul2, sul3, floR and strA resistance genes. For the first time, we identified a class 1 integron with the dfrA12-orfF-aadA2 gene cassette in S. Infantis. Using pulsed-field gel electrophoresis, we confirmed the presence of identical clusters of S. Agona, S. Enteritidis PT8, S. Infantis and S. Senftenberg in wastewater and black-headed gulls, thus indicating the possibility of resistant Salmonella isolates spreading over long distances in the environment.
摘要：
我们调查了来自布尔诺污水处理厂（WWTP）的沙门氏菌分离株的存在和流行病学相关性，捷克共和国和NoveMlyny自来水厂的黑头海鸥（Chroicocephalusridibundus）雏鸟，位于污水处理厂下游35公里处。2012年，我们收集了37个废水样本和284个海鸥泄殖腔拭子。从废水样本中，我们获得了属于19个血清型的89个沙门氏菌分离株。在这些样品的89%中包含至少一种抗性菌株。在38只幼海鸥中检测到10种不同血清型的沙门氏菌，其中14人(37%)对抗菌药物耐药。废水分离物大多对磺胺类和四环素具有抗性，海鸥分离到四环素和氨苄西林。我们检测到blaTEM-1，tet(A)，tet(B),tet(G),sul1、sul2、sul3、floR和strA抗性基因。第一次,我们在婴儿链球菌中鉴定出具有dfrA12-orfF-aadA2基因盒的1类整合子。使用脉冲场凝胶电泳，我们证实存在相同的S.Agona簇，美国肠炎PT8，S.Infantis和S.Senftenberg在废水和黑头海鸥中，因此表明耐药沙门氏菌分离株在环境中长距离传播的可能性。
