Abstract：
BACKGROUND: Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells.
METHODS: The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluorescent protein (copGFP). In this light, the resultant vector was constructed and used for transfection of Spodoptera frugiperda cells.
RESULTS: Expression of the copGFP protein in insect cells was confirmed by fluorescent microscopy and Western-blot analysis.
CONCLUSIONS: The application of copGFP control bacmid can be considered as an appropriate control for insect cell transfection.
METHODS: The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluorescent protein (copGFP). In this light, the resultant vector was constructed and used for transfection of Spodoptera frugiperda cells.
RESULTS: Expression of the copGFP protein in insect cells was confirmed by fluorescent microscopy and Western-blot analysis.
CONCLUSIONS: The application of copGFP control bacmid can be considered as an appropriate control for insect cell transfection.
摘要：
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