%0 Journal Article
%T A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (cop-GFP) in Baculovirus Expression System.
%A Shokrollahi N
%A Shahbazzadeh D
%A Pooshang-Bagheri K
%A Habibi-Anbouhi M
%A Jahanian-Najafabadi A
%A Behdani M
%J Iran Biomed J
%V 20
%N 3
%D Jul 2016
%M 26518237
暂无%R 10.7508/ibj.2016.03.008
%X <B>BACKGROUND: </B>Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells.<BR><B>METHODS: </B>The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluorescent protein (copGFP). In this light, the resultant vector was constructed and used for transfection of Spodoptera frugiperda cells.<BR><B>RESULTS: </B>Expression of the copGFP protein in insect cells was confirmed by fluorescent microscopy and Western-blot analysis.<BR><B>CONCLUSIONS: </B>The application of copGFP control bacmid can be considered as an appropriate control for insect cell transfection.