{Reference Type}: Journal Article
{Title}: A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (cop-GFP) in Baculovirus Expression System.
{Author}: Shokrollahi N;Shahbazzadeh D;Pooshang-Bagheri K;Habibi-Anbouhi M;Jahanian-Najafabadi A;Behdani M;
{Journal}: Iran Biomed J
{Volume}: 20
{Issue}: 3
{Year}: Jul 2016
暂无{DOI}: 10.7508/ibj.2016.03.008
{Abstract}: <B>BACKGROUND: </B>Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells.<BR><B>METHODS: </B>The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluorescent protein (copGFP). In this light, the resultant vector was constructed and used for transfection of Spodoptera frugiperda cells.<BR><B>RESULTS: </B>Expression of the copGFP protein in insect cells was confirmed by fluorescent microscopy and Western-blot analysis.<BR><B>CONCLUSIONS: </B>The application of copGFP control bacmid can be considered as an appropriate control for insect cell transfection.