Determination of concentration-time-mortality relationships versus LC50s according to OECD guideline 403.

• 发表时间: 1989
• 来源期刊: Exp Pathol
• DOI: 10.1016/s0232-1513(89)80015-5
• PMID: 2637172
• 文章类型: Journal Article
• 作者列表: Arts JH,Zwart A,Schoen ED,Klokman-Houweling JM

Mesh: Administration, Inhalation; Ammonia / administration & dosage toxicity; Animals; Chlorides / administration & dosage toxicity; Dose-Response Relationship, Drug; Female; Health Planning; Health Planning Guidelines; Hydrogen Sulfide / administration & dosage toxicity; Lung Diseases / chemically induced epidemiology; Male; Mortality; Rats; Rats, Inbred Strains; Risk Factors; Statistics as Topic; Time Factors

Abstract

The effect of decreasing the number of animals on the accuracy of estimated concentration-time-mortality relationships and the LC50 values derived from these was investigated by means of simulation methods. Studies of NH3, Cl2, H2S, and COCl2 were made using 5 rats per sex per group. Mortality rates were analysed in 500 new data sets obtained by randomly removing 4 rats/sex/group from the original data sets. It was concluded that the concentration-time-mortality relationship and the LC50 values can be determined over a 5-10-fold time range using one rat/sex/group. The resulting 5 and 95 percentiles compare favourably with the 90% confidence limits when determining a LC50 according to OECD guideline 403. When using concentration-time-mortality relationships, additional information can be obtained which can be used in inhalation hazard risk assessment.