■营养不良会引起氧化应激的增加,因为它会触发热休克蛋白70(HSP70)的表达,在最佳水平内修复受损细胞所需的伴侣分子。蜂蜜是一种可以刺激HSP70表达的饲料来源,可以在动物试验中给予营养不良的人。
■这项研究的目的是证明Kaliandra蜂蜜可以提高睾丸激素水平,直径,由于通过刺激HSP70而引起的营养不良,以及大鼠睾丸生精管(Rattusnorvegicus)的上皮厚度,该组织在免疫组织化学上表达。
■这项研究使用了40只雄性大鼠,将其分为四个治疗组:T0(阴性对照):正常大鼠,未给予蜂蜜;T1(阳性对照):营养不良大鼠,未给予蜂蜜;T2(治疗2):营养不良大鼠,给予30%卡丽安达蜂蜜(v/v)10天;T3(治疗3),营养不良的大鼠,并给予50%卡丽安德拉蜂蜜(v/v)10天。营养不良的状况是通过连续五天禁食饲料导致男性生殖器官受损来进行的,尤其是睾丸.
■结果表明,50%(v/v)剂量的卡利安德拉蜂蜜在改善睾丸激素水平方面具有显着作用,直径,通过刺激HSP70的表达,营养不良的雄性大鼠生精管的上皮厚度。通过IHC在T0、T1、T2和T3时的HSP70表达评分依次为0.15a±0.5、3.15c±0.4、2.95c±0.35和1.75b±0.15。酶联免疫吸附测定T0,T1,T2和T3的间接睾酮水平(以μg/dl为单位)依次为36.39c±0.35、6.12a±0.51、7.45a±0.15、25.27b±0.63。T0,T1,T2和T3四种处理的睾丸生精管的直径和上皮厚度(以μm为单位)依次为362.40c±4.71、248.46a±3.90、255.22a±2.34、318.37b±4.23和117.60d±11.30、3.86a±1.57、9.72b±3.96、29.84c±4.02。
■该研究的结论表明,50%(v/v)剂量的卡连德拉蜂蜜在改善睾丸激素水平方面具有显着的作用,直径,和通过HSP70刺激营养不良大鼠的生精小管的上皮厚度,尽管与阴性对照(T0)没有显着相同。
Malnutrition can cause an increase in oxidative stress as it triggers the expression of heat shock protein70 (HSP70), a chaperon molecule that is needed to repair damaged cells within optimal levels. Honey is a source of feed that can stimulate HSP70 expression, which can be given to the malnourished in the animal trial.
The purpose of this study was to prove that Kaliandra honey can improve testosterone levels, diameter, and epithelial thickness of the seminiferous tubule of rat testes (Rattus norvegicus) due to malnutrition through stimulation of HSP70, which is expressed immunohistochemically.
This study used 40 male rats, which were divided into four treatment groups: T0 (negative control): normal rats and not given honey; T1 (positive control): malnourished rats and not given honey; T2 (treatment 2): malnourished rats and given 30% Kaliandra honey (v/v) for 10 days; T3 (treatment 3), malnourished rats and given 50% Kaliandra honey (v/v) for 10 days. The condition of malnutrition is carried out by fasting the feed for five consecutive days resulting in damage to the male reproductive organs, especially the testes.
The results showed that Kaliandra honey at a dose of 50% (v/v) had a significant effect in improving testosterone levels, diameter, and epithelial thickness of seminiferous tubule of malnourished male rats through stimulation of HSP70 expression. The HSP70 expression scores by IHC at T0, T1, T2, and T3 were 0.15a ± 0.5, 3.15c ± 0.4, 2.95c ± 0.35, and 1.75b ± 0.15, sequentially. enzyme-linked immunosorbent assay indirect testosterone levels at T0, T1, T2, and T3 (in μg/dl) were 36.39c ± 0.35, 6.12a ± 0.51, 7.45a ± 0.15, 25.27b ± 0.63, sequentially. The diameter and epithelial thickness of the seminiferous tubule of the testes (in μm) in the four treatments T0, T1, T2, and T3 were 362.40c ± 4.71, 248.46a ± 3.90, 255.22a ± 2.34, 318.37b ± 4.23 and 117.60d ± 11.30, 3.86a ± 1.57, 9.72b ± 3.96, 29.84c ± 4.02 sequentially.
The conclusion of the study showed that Kaliandra honey at a dose of 50% (v/v) had a significant effect in improving testosterone levels, diameter, and epithelial thickness of the seminiferous tubule of malnourished rats through stimulation of HSP70, although not significantly the same as negative control (T0).