Abstract：
A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction. The enzyme, isolated from Thermus aquaticus, greatly simplifies the procedure and, by enabling the amplification reaction to be performed at higher temperatures, significantly improves the specificity, yield, sensitivity, and length of products that can be amplified. Single-copy genomic sequences were amplified by a factor of more than 10 million with very high specificity, and DNA segments up to 2000 base pairs were readily amplified. In addition, the method was used to amplify and detect a target DNA molecule present only once in a sample of 10(5) cells.
摘要：
热稳定的DNA聚合酶用于体外DNA扩增程序，聚合酶链反应.酶，从水热中分离出来，大大简化了程序，通过使扩增反应能够在更高的温度下进行，显著提高了特异性，产量，灵敏度,以及可以扩增的产物的长度。单拷贝基因组序列以非常高的特异性扩增超过1000万，和高达2000个碱基对的DNA片段容易被扩增。此外,该方法用于扩增和检测仅存在于10（5）个细胞样品中的靶DNA分子。
