小麦及其近缘种 WOX5 基因的鉴定与表达分析.
关键词: 1-naphthoxyacetic acid 2,4-D 2,4-dichlorophenoxyacetic acid 6-BA 6-benzyl-adenine AP BLAST CDA CIA CTAB EAR EC ERF-associated amphiphilic repression Expression HD IAA IPTG MS NAA NEC NJ ORF PAGE Phylogenetic analysis QHB RAM RT-qPCR Real-time quantitative PCR SAM SDS SNPs TAIL-PCR USDA-ARS UTR United States Department of Agriculture—Agricultural Research Service WOX WOX5 WUS WUS-related homeobox Wheat Wuschel adaptor primer basic local alignment search tool callus in differentiation medium after days callus in induced medium after days cetyltrimethyl ammonium bromide embryogenic callus homeodomain indole-3-acetic acid isopropyl β-d-thiogalactopyranoside murashige and skoog medium neighbor-joining non-embryogenic callus open reading frame polyacrylamide gel electrophoresis quiescent center-specific homeobox real-time quantitative PCR root apical meristem shoot apical meristem single nucleotide polymorphism sodium dodecyl sulfate thermal asymmetric interlaced PCR untranslated transcribed region
Mesh : Amino Acid Sequence Escherichia coli / genetics Gene Expression Regulation, Plant Genes, Homeobox Indoleacetic Acids / metabolism Molecular Sequence Data Multigene Family Open Reading Frames Phylogeny Plant Proteins / genetics metabolism Plant Roots / genetics Poaceae / genetics Protein Structure, Tertiary Triticum / genetics
来 源:
DOI:10.1016/j.gene.2013.12.022
PDF(Sci-hub)