GATA转录因子,它们是具有IV型锌指结合结构域的DNA结合蛋白,在生物有机体的转录调控中发挥作用。它们在植物的生长发育中起着不可或缺的作用,以及提高他们面对各种环境压力的能力。迄今为止,已经在许多基因家族中发现了GATAs,但是在以前的探索中,龙眼中的GATA基因(DimocarpuslonganLour)尚未被研究。龙眼GATA家族基因的各个方面,包括它们的识别和分类,它们在染色体上的位置分布,它们的外显子/内含子结构,同质性分析,它们在不同温度下的表达,PEG的浓度,体细胞胚的早期发育阶段及其在不同组织中的表达水平,和外源激素的浓度,在这项研究中进行了调查。这项研究表明,22个DlGATA可以分为四个亚家族。在DlGATA和AtGATA的同系性分析中,有10对同源GATA基因。DLGATA家族成员中存在四个分段复制基序和一对串联复制事件。还发现位于启动子区域的顺式作用元件富含光响应元件,其中含有相关的激素反应性元素。在体细胞胚胎中,DlGATA4在球状胚(GE)阶段的表达上调。我们还发现DLGATA表达在根和茎中强烈上调。研究表明,在激素(ABA和IAA)处理下,DlGATA在龙眼胚性愈伤组织中的表达。在ABA处理下,DlGATA4上调,其他DlGATA基因无明显反应。此外,如qRT-PCR所示,在100μmol·L-1浓度IAA处理下,DlGATA基因的表达表现出强烈的上调表达水平。该实验进一步研究了这些,并模拟了它们与干旱响应机制的可能联系,同时将它们与PEG处理下的表达相关联。总的来说,这个实验探索了GATA基因并深入研究了它们的进化,结构,函数,和表达谱,从而为更深入地研究GATA家族的基因特征提供更多的信息。
GATA transcription factors, which are DNA-binding proteins with type IV zinc finger binding domains, have a role in transcriptional regulation in biological organisms. They have an indispensable role in the growth and development of plants, as well as in improvements in their ability to face various environmental stresses. To date, GATAs have been identified in many gene families, but the GATA gene in longan (Dimocarpus longan Lour) has not been studied in previous explorations. Various aspects of genes in the longan GATA family, including their identification and classification, the distribution of their positions on chromosomes, their exon/intron structures, a synteny analysis, their expression at different temperatures, concentration of PEG, early developmental stages of somatic embryos and their expression levels in different tissues, and concentrations of exogenous hormones, were investigated in this study. This study showed that the 22 DlGATAs could be divided into four subfamilies. There were 10 pairs of homologous GATA genes in the synteny analysis of DlGATA and AtGATA. Four segmental replication motifs and one pair of tandem duplication events were present among the DlGATA family members. The cis-acting elements located in promoter regions were also found to be enriched with light-responsive elements, which contained related hormone-responsive elements. In somatic embryos, DlGATA4 is upregulated for expression at the globular embryo (GE) stage. We also found that DlGATA expression was strongly up-regulated in roots and stems. The study demonstrated the expression of DlGATA under hormone (ABA and IAA) treatments in embryogenic callus of longan. Under ABA treatment, DlGATA4 was up-regulated and the other DlGATA genes did not respond significantly. Moreover, as demonstrated with qRT-PCR, the expression of DlGATA genes showed strong up-regulated expression levels under 100 μmol·L-1 concentration IAA treatment. This experiment further studied these and simulated their possible connections with a drought response mechanism, while correlating them with their expression under PEG treatment. Overall, this experiment explored the GATA genes and dug into their evolution, structure, function, and expression profile, thus providing more information for a more in-depth study of the characteristics of the GATA family of genes.