关键词: 1-anilinonaphtalene-8-sulfonate ADCC ANS Autosomal dominant congenital nuclear cataract BSA C-terminal domain CD CTD DTT E(max) GdnHCl IPTG Inherited mutation MD MW Molecular dynamic simulation Protein aggregation Protein assembly SDS SDS-PAGE SDS-polyacrylamide gel electrophoresis SEC T(m) WT autosomal dominant congenital cataract bovine serum albumin circular dichroism dithiothreitol guanidine hydrochloride isopropyl-1-thio-β-d-galactopyranoside maximum emission wavelength of intrinsic Trp fluorescence molecular dynamics molecular weight size-exclusion chromatography sodium dodecyl sulfate the midpoint temperature of protein thermal transition wild type βB2-crystallin
Mesh : Amino Acid Sequence Escherichia coli / genetics metabolism Humans Hydrogen Bonding Hydrophobic and Hydrophilic Interactions Kinetics Lens, Crystalline / chemistry Molecular Dynamics Simulation Molecular Sequence Data Mutation Protein Folding Protein Multimerization Protein Stability Protein Structure, Secondary Protein Structure, Tertiary Recombinant Proteins / chemistry genetics Solubility beta-Crystallin B Chain / chemistry genetics
来 源:
DOI:10.1016/j.bbadis.2013.10.001
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