氧化铁纳米颗粒(IONP)在生物医学领域得到了广泛的探索,生物传感,热疗,和药物/基因传递,归因于它们的多功能和可调属性。然而,由于其众多的应用,需要用适当的材料对IONP进行功能化。为了实现IONP的最佳功能化,聚多巴胺(PDA)由于其提供优异的功能化表面的能力而被利用,近红外光吸收,和粘合剂性质定制所需的功能化IONP。这种涉及PDA的概念导致了磁铁矿-PDA纳米颗粒的成功合成,其中PDA表面涂覆在磁铁矿(Fe3O4@PDA)上。使用TEM等技术对Fe3O4@PDA纳米粒子进行了表征,FESEM,PXRD,XPS,VSM,和FTIR,表明PDA成功附着磁铁矿晶体结构保留。人血清白蛋白(HSA),血浆中的主要蛋白质,与递送的纳米粒子相互作用。因此,我们采用了各种光谱技术,随着细胞毒性,考察了Fe3O4@PDANPs对HSA稳定性和结构的影响。使用圆二色性(CD)和同步荧光光谱法(SFS)检查了结构变化。已经观察到,在与Fe3O4@PDA相互作用后,HSA蛋白的二级结构没有结构扰动。使用稳态荧光的研究表明,与Fe3O4@PDA相互作用后,HSA的固有荧光强度受到抑制。此外,温度相关的荧光测量表明,猝灭的类型包括同时静态和动态猝灭。对果蝇幼虫的细胞毒性研究显示没有细胞毒性作用,但仅在较高浓度下显示出较小的遗传毒性作用。
Iron oxide nanoparticles (IONPs) have been extensively explored in biomedicine, bio-sensing, hyperthermia, and drug/gene delivery, attributed to their versatile and tunable properties. However, owing to its numerous applications, the functionalization of IONPs with appropriate materials is in demand. To achieve optimal functionalization of IONPs, polydopamine (PDA) was utilized due to its ability to provide a superior functionalized surface, near-infrared light absorption, and adhesive nature to customize desired functionalized IONPs. This notion of involving PDA led to the successful synthesis of magnetite-PDA nanoparticles, where PDA is surface-coated on magnetite (Fe3O4@PDA). The Fe3O4@PDA nanoparticles were characterized using techniques like TEM, FESEM, PXRD, XPS, VSM, and FTIR, suggesting PDA\'s successful attachment with magnetite crystal structure retention. Human serum albumin (HSA), the predominant protein in blood plasma, interacts with the delivered nanoparticles. Therefore, we have employed various spectroscopic techniques, along with cytotoxicity, to inspect the effect of Fe3O4@PDA NPs on the stability and structure of HSA. The structural alterations were examined using circular dichroism (CD) and synchronous fluorescence spectroscopy (SFS). It has been observed that there are no structural perturbations in the secondary structure of the HSA protein after interaction with Fe3O4@PDA. Studies using steady-state fluorescence revealed that the inherent fluorescence intensities of HSA were suppressed after interaction with Fe3O4@PDA. In addition, temperature-dependent fluorescence measurements suggested that the type of quenching consists of both static and dynamic quenching simultaneously. A cytotoxicity study in Drosophila melanogaster larvae revealed no cytotoxic effects but did show a minor genotoxic effect only at higher concentrations.