Abstract：
Leiomyosarcoma (LMS) is a relatively uncommon malignant tumour derived from smooth muscle cells that rapidly metastasizes to distant regions. It rarely reaches oral tissues in which smooth muscle tissues are absent. We report the case of a 56-year-old woman who presented with LMS in the maxilla that had metastasized from a primary tumour in her uterus, received a total hysterectomy with bilateral salpingo-oophorectomy 9 months earlier. To reveal the poor prognosis of metastatic LMS, a total of 26 antibodies against different factors related to the proliferation, apoptosis, necrosis, and angiogenesis were simultaneously applied on the immunohistochemistry and immuno-blot detection in order to screen for expression n of different proteins in the metastatic LMS. Compared with the immunoreactions of primary uterine LMS, the different antibodies for cellular proliferation, i.e., proliferating cell nuclear antigen (PCNA), multiple primary neoplasm-2 (MPN-2), Max, p21, CDK4, p53, Rb-1, Bad, Bcl-2, epidermal growth factor receptor (EGF-R), hepatocyte growth factor (HGF), C-erbb2, Maspin, and DMBT-1, and those for angiogenesis, i.e., vWF, CD31, and Angiogenin, were more intensely expressed, while Bax, p16, Wnt-1, E-cadherin, and APC were relatively weakly expressed. In particular, beta-catenin was densely localized to the nuclei of tumour cells. These data suggest that rapid proliferation of the tumour cells is related to over-expression of different oncogenes, and that the infiltrative growth and early distant metastasis of these tumour cells are related to over-expression of angiogenesis factors. A total of seven cases of metastatic LMS to the oral cavity that had been published in the English literature were reviewed, and the reason for the poor prognosis in the metastatic LMS is suggested in this case report.
摘要：
平滑肌肉瘤（LMS）是一种相对罕见的恶性肿瘤，源自平滑肌细胞，可迅速转移到远处。它很少到达没有平滑肌组织的口腔组织。我们报告了一名56岁的女性，该女性在上颌骨中出现LMS，该患者从子宫中的原发性肿瘤转移而来，9个月前接受了全子宫切除术和双侧附件卵巢切除术。为了揭示转移性LMS的不良预后，共有26种抗体针对与增殖相关的不同因素，凋亡，坏死,将血管生成同时应用于免疫组织化学和免疫印迹检测，以筛选转移LMS中不同蛋白的表达。与原发性子宫LMS的免疫反应相比，细胞增殖的不同抗体，即，增殖细胞核抗原(PCNA),多原发肿瘤-2（MPN-2），麦克斯,p21,CDK4,p53,Rb-1,坏,Bcl-2,表皮生长因子受体(EGF-R),肝细胞生长因子（HGF），C-erbb2,Maspin,和DMBT-1，以及用于血管生成的那些，即，vWF,CD31和血管生成素，表达更强烈，而Bax,p16,Wnt-1,E-钙黏着蛋白,和APC表达相对较弱。特别是,β-连环蛋白密集地定位于肿瘤细胞的细胞核。这些数据表明，肿瘤细胞的快速增殖与不同癌基因的过度表达有关，这些肿瘤细胞的浸润生长和早期远处转移与血管生成因子的过度表达有关。回顾了英语文献中发表的7例口腔转移性LMS病例，并在此病例报告中提出了转移性LMS预后不良的原因。
