背景:结肠直肠癌(CRC)是一种被认为受人乳头瘤病毒(HPV)和人多瘤病毒(HPyVs)影响的癌症类型。在埃及,CRC是第7位最常见的癌症,占男性癌症的3.47%和女性癌症的3%。然而,目前缺乏有关埃及CRC病例中PyVs和HPVs共感染的信息。因此,本研究的目的是调查HPV和HPyV的发生(JCPyV,BKPyV,和SV40)感染,以及共同感染,在埃及的CRC患者中。此外,该研究旨在评估这些病毒感染与肿瘤分期之间的任何潜在关联。
方法:在本研究中,我们分析了来自埃及CRC患者的51个组织样本,还有19个息肉样本。我们的研究重点是使用Real-TimePCR对HPyV进行检测和基因分型。此外,我们采用实时PCR检测HPV,以及他们的基因分型,我们使用了PCR扩增和测序的组合。
结果:在我们的研究中,我们在CRC患者中发现了HPyVs感染的证据,特别是SV40(25.5%)和BKPyV(19.6%)。然而,在检查的样品中未检测到JCPyV。此外,我们发现HPV存在于43.1%的CRC患者中.当考虑病毒感染时,19.6%的CRC样本显示多种病毒共存,而在息肉样本中没有发现共感染。重要的是,我们观察到HPV的存在与晚期结直肠肿瘤B2级和D级之间存在显著相关性。
结论:我们的研究结果为结直肠癌(CRC)中致癌病毒的检测提供了有价值的数据,并强调了病毒共同感染与晚期肿瘤分期的相关性.然而,有必要对更大队列进行进一步研究,以验证这些发现并加强其在CRC领域的重要性.
BACKGROUND: Colorectal cancer (CRC) is a cancer type that is thought to be influenced by human papillomaviruses (HPVs) and human polyomaviruses (HPyVs). In Egypt, CRC ranks as the 7th most common cancer, accounting for 3.47% of male cancers and 3% of female cancers. However, there is currently a lack of information regarding the presence of PyVs and HPVs co-infection specifically in CRC cases in Egypt. Therefore, the aim of this study was to investigate the occurrence of HPVs and HPyVs (JCPyV, BKPyV, and SV40) infections, as well as co-infections, among CRC patients in Egypt. Additionally, the study aimed to assess any potential association between these viral infections and tumor stages.
METHODS: In the present study, we analyzed a total of 51 tissue samples obtained from Egyptian CRC patients, along with 19 polyps\' samples. Our investigation focused on the detection and genotyping of HPyVs using Real-Time PCR. Additionally, we employed real-time PCR for the detection of HPVs, and for their genotyping, we utilized a combination of PCR amplification followed by sequencing.
RESULTS: In our study, we found evidence of HPyVs infection in the CRC patients, specifically SV40 (25.5%) and BKPyV (19.6%). However, JCPyV was not detected in the samples that were examined. Additionally, we discovered that
HPV was present in 43.1% of the CRC patients. When considering viral co-infections, 19.6% of the CRC samples showed coexistence of multiple viruses, while no co-infections were found in the polyps samples. Importantly, we observed a significant correlation between the presence of HPVs and advanced colorectal tumor grades B2 and D.
CONCLUSIONS: Our findings provide valuable data for the detection of oncogenic viruses in colorectal cancer (CRC) and underscore the association of viral co-infections with advanced tumor stages. However, further research with larger cohorts is necessary to validate these findings and strengthen their significance in the field of CRC.