背景:纳米技术在农业领域具有革命性的潜力,氧化锌纳米颗粒(ZnONPs)在促进作物生长方面表现出优势。光合效率的提高与茶树的活力和优良品质密切相关。辅以叶层微生物在维持植物健康方面的有益作用。然而,ZnONPs对茶树光合作用的影响,新芽的发芽,叶球微生物群落尚未得到充分研究。
结果:本研究调查了ZnONPs对茶树光合生理参数的影响,关键光合酶的含量,如RubisCO,叶绿素含量,叶绿素荧光参数,叶片和新芽的转录组学和广泛的靶向代谢组学概况,这些组织中的矿物元素组成,以及叶球内的附生和内生微生物群落。结果表明,ZnONPs可以增强茶树的光合作用,上调与光合作用相关的一些基因的表达,增加光合产物的积累,促进新芽的发展,改变茶树叶片和新芽中各种矿质元素的含量。此外,观察到ZnONPs的应用有利地影响了茶树叶球内的微生物群落结构。微生物群落动力学的这种变化表明,ZnONPs有可能通过调节叶球微生物组来促进植物健康和生产力。
结论:这项研究表明,ZnONPs对茶树的光合作用有积极的影响,新芽的发芽,和叶际微生物群落,可以改善茶树的生长状况。这些发现为ZnONPs在可持续农业发展中的应用提供了新的科学证据,并有助于推进旨在提高作物产量和质量的纳米生物技术研究。
BACKGROUND: Nanotechnology holds revolutionary potential in the field of agriculture, with zinc oxide nanoparticles (ZnO NPs) demonstrating advantages in promoting crop growth. Enhanced photosynthetic efficiency is closely linked to improved vigor and superior quality in tea plants, complemented by the beneficial role of phyllosphere microorganisms in maintaining plant health. However, the effects of ZnO NPs on the photosynthesis of tea plants, the sprouting of new shoots, and the community of phyllosphere microorganisms have not been fully investigated.
RESULTS: This study investigated the photosynthetic physiological parameters of tea plants under the influence of ZnO NPs, the content of key photosynthetic enzymes such as RubisCO, chlorophyll content, chlorophyll fluorescence parameters, transcriptomic and extensive targeted metabolomic profiles of leaves and new shoots, mineral element composition in these tissues, and the epiphytic and endophytic microbial communities within the phyllosphere. The results indicated that ZnO NPs could enhance the photosynthesis of tea plants, upregulate the expression of some genes related to photosynthesis, increase the accumulation of photosynthetic products, promote the development of new shoots, and alter the content of various mineral elements in the leaves and new shoots of tea plants. Furthermore, the application of ZnO NPs was observed to favorably influence the microbial community structure within the phyllosphere of tea plants. This shift in microbial community dynamics suggests a potential for ZnO NPs to contribute to plant health and productivity by modulating the phyllosphere microbiome.
CONCLUSIONS: This study demonstrates that ZnO NPs have a positive impact on the photosynthesis of tea plants, the sprouting of new shoots, and the community of phyllosphere microorganisms, which can improve the growth condition of tea plants. These findings provide new scientific evidence for the application of ZnO NPs in sustainable agricultural development and contribute to advancing research in nanobiotechnology aimed at enhancing crop yield and quality.