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Abstract：
In the present study, zinc oxide nanoparticles (ZnO-NPs) were synthesized using neem leaf aqueous extracts and characterized using transmission electron microscopy (TEM), ultraviolet visible spectroscopy (UV-Vis), and dynamic light scattering (DLS). Then compare its efficacy as anticancer and antibacterial agents with chemically synthesized ZnO-NPs and the neem leaf extract used for the green synthesis of ZnO-NPs. The TEM, UV-vis, and particle size confirmed that the developed ZnO-NPs are nanoscale. The chemically and greenly synthesized ZnO-NPs showed their optical absorbance at 328 nm and 380 nm, respectively, and were observed as spherical particles with a size of about 85 nm and 62.5 nm, respectively. HPLC and GC-MS were utilized to identify the bioactive components in the neem leaf aqueous extract employed for the eco-friendly production of ZnO-NPs. The HPLC analysis revealed that the aqueous extract of neem leaf contains 19 phenolic component fractions. The GC-MS analysis revealed the existence of 21 bioactive compounds. The antiproliferative effect of green ZnO-NPs was observed at different concentrations (31.25 µg/mL-1000 µg/mL) on Hct 116 and A 549 cancer cells, with an IC50 value of 111 µg/mL for A 549 and 118 µg/mL for Hct 116. On the other hand, the antibacterial activity against gram-positive and gram-negative bacteria was estimated. The antibacterial result showed that the MIC of green synthesized ZnO-NPs against gram-positive and gram-negative bacteria were 5, and 1 µg/mL. Hence, they could be utilized as effective antibacterial and antiproliferative agents.
摘要：
在本研究中,氧化锌纳米颗粒（ZnO-NPs）是使用苦参叶水提物合成的，并使用透射电子显微镜（TEM）进行表征，紫外可见光谱(UV-Vis),和动态光散射(DLS)。然后将其作为抗癌和抗菌剂的功效与化学合成的ZnO-NPs和用于绿色合成ZnO-NPs的印em叶提取物进行比较。TEM，UV-vis,和粒径证实了开发的ZnO-NP是纳米级的。化学和绿色合成的ZnO-NP在328nm和380nm处显示出其吸光度，分别,并观察到球形颗粒的大小约为85nm和62.5nm，分别。利用HPLC和GC-MS鉴定了用于环保型ZnO-NP生产的印em叶水提物中的生物活性成分。HPLC分析显示，印度苦艾叶的水提取物含有19种酚类成分。GC-MS分析显示存在21种生物活性化合物。在不同浓度（31.25µg/mL-1000µg/mL）下观察到绿色ZnO-NP对Hct116和A549癌细胞的抗增殖作用，A549的IC50值为111µg/mL，Hct116的IC50值为118µg/mL。另一方面,评估了对革兰氏阳性和革兰氏阴性细菌的抗菌活性。结果表明，绿色合成的ZnO-NP对革兰氏阳性菌和革兰氏阴性菌的MIC分别为5和1µg/mL。因此，它们可以用作有效的抗菌和抗增殖剂。
