■尽管呼吸道中念珠菌的存在通常被认为是共生的,在免疫受损或COVID-19患者中认识念珠菌定植的重要性至关重要.耳念珠菌作为一种新兴病原体的出现进一步强调了监测酵母菌感染/定植的重要性,特别是在COVID-19患者中。
■在这项研究中,呼吸道样本主要来自COVID-19患者,主要是那些怀疑有真菌感染的人,在Sabouraud葡萄糖琼脂平板上培养,并使用两步多重PCR方法鉴定酵母菌落。对怀疑为C.auris的样品进行特异性巢式PCR,随后进行序列分析。
■共收集了来自73名女性和126名男性的199个呼吸道样本,年龄从1.6岁到88岁不等。在患者中,141人患有COVID-19,32人患有癌症,5人在ICU住院,2例患有慢性阻塞性肺疾病)COPD(,其他人是合并疾病的患者。从这些样本中,共鉴定出334株酵母菌株。白色念珠菌(n=132,39.52%)是最常见的物种,其次是热带梭菌(n=67,20%),C.光滑(n=56,16.76%),C.krusei(n=18,5.4%),C.近平滑(n=17,5.08%),酿酒酵母(n=10,3%),C.kefyr(n=9,2.6%),C.都柏林人(n=7,2.1%),C.lusitaniae(n=5,1.5%),C.auris(n=3,0.9%),C.guilliermondii(n=2,0.6%),C.rugosa(n=1,0.3%),C.中间(n=1,0.3%),和Trichosporonspp。(n=1,0.3%)。在ICU的一名患者和两名COVID-19患者中检测到金黄色葡萄球菌。虽然通过序列分析证实了它的存在,我们广泛的分离C.auris的努力没有成功.
■虽然白色念珠菌定植仍然很普遍,我们的研究没有发现念珠菌肺部感染的证据.由于研究有限,念珠菌定植在气道分泌物中的作用仍然不明确,进一步的研究必须阐明这个问题。
UNASSIGNED: Although the existence of Candida species in the respiratory tract is often considered commensal, it is crucial to recognize the significance of Candida colonization in immunocompromised or COVID-19 patients. The emergence of Candida auris as an emerging pathogen further emphasizes the importance of monitoring yeast infection/colonization, particularly in COVID-19 patients.
UNASSIGNED: In this study, respiratory samples mainly from COVID-19 patients, primarily those suspected of having a fungal infection, were cultured on Sabouraud dextrose agar plates and the yeast colonies were identified using a two-step multiplex PCR method. The samples suspected of C. auris underwent specific nested PCR followed by sequence analysis.
UNASSIGNED: A total of 199 respiratory samples were collected from 73 women and 126 men, ranging in age from 1.6 to 88 years. Among the patients, 141 had COVID-19, 32 had cancer, 5 were hospitalized in ICU, 2 had chronic obstructive pulmonary disease)COPD(, and others were patients with combination diseases. From these samples, a total of 334 yeast strains were identified. C. albicans (n=132, 39.52%) was the most common species, followed by C. tropicalis (n=67, 20%), C. glabrata (n=56, 16.76%), C. krusei (n=18, 5.4%), C. parapsilosis (n=17, 5.08%), Saccharomyces cerevisiae (n=10, 3%), C. kefyr (n=9, 2.6%), C. dubliniensis (n=7, 2.1%), C. lusitaniae (n=5, 1.5%), C. auris (n=3, 0.9%), C. guilliermondii (n=2, 0.6%), C. rugosa (n=1, 0.3%), C. intermedia (n=1, 0.3%), and Trichosporon spp. (n=1, 0.3%). C. auris was detected in a patient in ICU and two COVID-19 patients. While its presence was confirmed through sequence analysis, our extensive efforts to isolate C. auris were unsuccessful.
UNASSIGNED: While C. albicans colonization remains prevalent, our study found no evidence of Candida lung infection. Since the role of Candida colonization in airway secretions remains ambiguous due to limited research, further studies are imperative to shed light on this matter.