关键词: Hanseniaspora menglaensis rotting wood yeast species

Mesh : Hanseniaspora / genetics Phylogeny Wood China DNA, Fungal / genetics Mycological Typing Techniques Sequence Analysis, DNA DNA, Ribosomal Spacer / genetics Base Composition RNA, Ribosomal, 16S / genetics DNA, Bacterial / genetics Bacterial Typing Techniques Fatty Acids / chemistry Saccharomycetales

来  源:   DOI:10.1099/ijsem.0.005970

Abstract:
Two apiculate strains (NYNU 181072 and NYNU 181083) of a bipolar budding yeast species were isolated from rotting wood samples collected in Xishuangbanna Tropical Rainforest in Yunnan Province, southwest PR China. On the basis of phenotypic characteristics and the results of phylogenetic analysis of the D1/D2 domain of the large subunit (LSU) rRNA, internal transcribed spacer (ITS) region and the actin (ACT1) gene, the two strains were found to represent a single novel species of the genus Hanseniaspora, for which the name Hanseniaspora menglaensis f.a., sp. nov. (holotype CICC 33364T; MycoBank MB 847437) is proposed. In the phylogenetic tree, H. menglaensis sp. nov. showed a close relationship with Hanseniaspora lindneri, Hanseniaspora mollemarum, Hanseniaspora smithiae and Hanseniaspora valbyensis. H. menglaensis sp. nov. differed from H. lindneri, the most closely related known species, by 1.2 % substitutions in the D1/D2 domain, 2.5 % substitutions in the ITS region and 5.4 % substitutions in the ACT1 gene, respectively. Physiologically, H. menglaensis sp. nov. can also be distinguished from H. lindneri by its ability to assimilate d-gluconate.
摘要:
从云南省西双版纳热带雨林中收集的腐烂木材样品中分离出两株双极出芽酵母菌种(NYNU181072和NYNU181083),西南公关中国。根据大亚基(LSU)rRNA的D1/D2结构域的表型特征和系统发育分析结果,内部转录间隔区(ITS)和肌动蛋白(ACT1)基因,发现这两个菌株代表了Hanseniasspora属的一个新物种,Hanseniasporamenglaensisf.a.,sp.11月。(holotypeCICC33364T;MycoBankMB847437)被提议。在系统发育树中,H.menglaensissp.11月。与Hanseniassporalindneri关系密切,Hanseniassporamolemarum,汉森孢子和汉森孢子。H.menglaensissp.11月。不同于H.Lindneri,最密切相关的已知物种,通过D1/D2结构域中的1.2%取代,在ITS区域有2.5%的取代,在ACT1基因中有5.4%的取代,分别。生理学上,H.menglaensissp.11月。也可以通过其吸收d-葡萄糖酸盐的能力与H.lindneri区分开。
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