糖尿病是伤口愈合中精心安排的事件的破坏因素之一。这就需要有针对性的药物,不断由纳米材料提供。在这里,我们提出了绿色合成的氧化铜纳米颗粒(CuONPs),从石榴(PG)或番石榴(GV)提取物中获得,作为有效的杀菌和杀真菌材料,促进目标糖尿病损伤组织的再生和愈合。
■比较了PG或GV植物提取物作为CuONP合成过程的还原剂来源。比较了产率和光催化降解潜力。从优越的提取物中获得的NPs,PG,用颗粒大小来表征,zeta电位,XRD,TEM,SEM,EDX对多重耐药的人类病原体评估了抗菌作用,然后确定了生物膜抑制浓度的百分比。在正常人皮肤细胞系上进行细胞毒性和创伤划痕研究。评估了糖尿病大鼠的体内伤口愈合活性,并对CD45和α-SMA进行了组织病理学和免疫组织化学检查。
■绿色合成的CuONP是直径为233nm的球形。CuONPs(250µg/mL)是针对多种耐多药人类病原体的有前途的生物防治剂。与对照仅2.001±0.155%相比,它们显着显示出刮伤伤口的29.460±0.811%愈合。伤口愈合实验揭示了低CuONP浓度在糖尿病动物模型以及人正常皮肤成纤维细胞系中的安全性。剂量为2mg/cm2的治疗组显示出优异的结果,WC50值为7.2天,13天后伤口收缩92%。同一组的免疫组织化学检查显示已建立的纤维组织(5.7±3.7/HPF),和最近发育的血管的肉芽组织扩增(70±1.5/HPF)。
■Green合成的CuONPs可以克服耐药性,有效促进伤口愈合过程。
UNASSIGNED: Diabetes mellitus is among the disrupting factors of orchestrated events in wound healing. This necessitates the urge for tailored medications, which are continually offered by nano-sized materials. Herein, we present greenly synthesized copper oxide nanoparticles (CuO NPs), obtained from either Punica granatum L. (PG) or Pisidium guajava L. (GV) extract, to function as potent bactericidal and fungicidal materials that promote regeneration and healing of the targeted diabetic wounded tissues.
UNASSIGNED: PG or GV plant extracts were compared as source of reducing agents for CuO NPs synthesis process. The yield and photocatalytic degradation potential were compared. NPs obtained from the superior extract, PG, were characterized using particles size, zeta potential, XRD, TEM, SEM, and EDX. The antimicrobial effects were evaluated on multidrug-resistant human pathogens and then the percentage biofilm inhibitory concentration was determined. The cytotoxicity and wound scratch study were conducted on a normal human skin cell line. In-vivo wound healing activity in diabetic rats was assessed along with histopathological and immunohistochemical examination of CD45 and α-SMA.
UNASSIGNED: The greenly synthesized CuO NPs are spherical in shape having a diameter of 233nm. CuO NPs (250µg/mL) acted as promising biocontrol agent against a variety of multidrug-resistant human pathogens. They significantly exhibited 29.460±0.811% healing of the scratched wound compared to only 2.001±0.155% for the control. Wound healing experiments revealed the safety of a low CuO NPs concentration in a diabetic animal model as well as on human normal skin fibroblast cell line. The treated group with a dose of 2mg/cm2 showed superior results with a WC50 value of 7.2 days, and 92% wound contraction after 13-days. Immunohistochemical investigation of the same group demonstrated well-established fibrous tissue (5.7±3.7/HPF), and an amplified granulation tissue of recently developed blood vessels (70±1.5/HPF).
UNASSIGNED: Green synthesized CuO NPs could overcome drug resistance and promote wound healing process effectively.