Abstract：
Hydrogels are commonly used as carriers for cell delivery due to their similarities to the extracellular matrix. A contraction-suppressed full-thickness wound model was used to evaluate the therapeutic potential of Pluronic F127 (PF127) hydrogel loaded with adipose-derived stromal vascular fraction (AdSVF), mesenchymal stem cells (AdMSC), and conditioned media (AdMSC-CM) for the repair of wounds in a rabbit model. The experimental study was conducted on forty-eight healthy adult New Zealand white rabbits randomly divided into eight groups with six animals each and treated with AdSVF, AdMSC, and AdMSC-CM as an injectable or topical preparation. The healing potential of different adipose-derived cell-based and cell-free therapeutics was evaluated based on percentage wound healing, period of epithelialization, epidermal thickness, scar evaluation, histopathology analysis, histochemical evaluation, immunohistochemistry (collagen type I), and hydroxyproline assay by comparing with the positive and negative control. Collagen density analysis using different staining methods, immunohistochemistry, and hydroxyproline assay consistently showed that delivering AdMSC and AdMSC-CM in PF127 hydrogel enhanced epithelialization, collagen production, and organization, contributing to improved tissue strength and quality. Even though allogeneic AdSVF was found to promote wound healing in rabbits, it has a lower potential than AdMSC and AdMSC-CM. The wound healing potential of AdMSC and AdMSC-CM was enhanced when loaded in PF127 hydrogel and applied topically. Even though wounds treated with AdMSC outperformed AdMSC-CM, a significant difference in the healing quality was not observed in most instances, indicating almost similar therapeutic potential. The findings indicate that the wound healing potential of AdMSC and AdMSC-CM was enhanced when loaded in PF127 hydrogel and applied topically. These treatments promoted collagen production, tissue organization, and epidermal regeneration, ultimately improving overall healing outcomes.
摘要：
水凝胶由于其与细胞外基质的相似性而通常用作细胞递送的载体。使用收缩抑制的全层伤口模型来评估负载有脂肪来源的基质血管分数（AdSVF）的PluronicF127（PF127）水凝胶的治疗潜力，间充质干细胞（AdMSC），和条件培养基(AdMSC-CM)用于修复兔模型中的伤口。本实验用健康成年新西兰大白兔48只,随机分为8组,每组6只,用AdSVF处理,AdMSC,和AdMSC-CM作为可注射或局部制剂。基于伤口愈合百分比评估了不同脂肪来源的细胞和无细胞疗法的愈合潜力，上皮形成期，表皮厚度,疤痕评估，组织病理学分析,组织化学评估，免疫组织化学（I型胶原），与阳性和阴性对照比较，测定羟脯氨酸。使用不同的染色方法进行胶原蛋白密度分析，免疫组织化学，和羟脯氨酸测定一致表明，在PF127水凝胶中递送AdMSC和AdMSC-CM可增强上皮形成，胶原蛋白生产，和组织，有助于提高组织强度和质量。即使发现同种异体AdSVF可以促进兔子的伤口愈合，它具有比AdMSC和AdMSC-CM更低的潜力。当加载到PF127水凝胶中并局部应用时,AdMSC和AdMSC-CM的伤口愈合潜力增强。尽管用AdMSC治疗的伤口优于AdMSC-CM，在大多数情况下，没有观察到愈合质量的显着差异，表明几乎相似的治疗潜力。研究结果表明，当加载到PF127水凝胶中并局部应用时，AdMSC和AdMSC-CM的伤口愈合潜力得到增强。这些治疗促进了胶原蛋白的产生，组织组织,和表皮再生，最终改善整体愈合结果。
