以前的工作表明,饮食治疗对木质乳房(WB)发病率的影响不同,这表明肠道条件,如肠道屏障功能,炎症,氧化应激可能与WB有关。在这项研究中,研究了膳食补充抗生素(杆菌肽)或益生菌(枯草芽孢杆菌)对肠道屏障功能相关转录物表达的影响,炎症,和有或没有WB的肉鸡空肠粘液衬里的氧化应激。在这项研究中使用了分裂图实验设计。饮食处理是主要的图因子,而胸肌状况是子图因子。在第41天,从表现出WB的每3个饮食处理组中的3个重复围栏中的每一个的1只鸟和含有正常乳房的另外的鸟收集空肠粘液(3个生物学重复/处理/表型;3×3×2,总N=18)。使用商业RNA提取试剂盒提取总RNA。CLDN1、MUC6、TLR2A的表达水平,TLR2B,TLR4、IFN-γ、IL-1β,使用两步RT-qPCR分析测定IL-8L1、IL-10、NOS2和SOD。在用鸡18SrRNA基因归一化后测定ΔCt值的基因表达差异。当治疗之间出现显著差异时,使用ΔΔCt方法计算相对倍数变化并计算显著性水平。使用SAS9.4的PROCGLM程序,显著性水平设定为P≤0.05。饮食和胸肌状况对所测试的任何基因的表达之间没有显著的交互作用。然而,与正常胸肌的鸟类相比,具有WB的鸟类表现出更高的MUC6基因表达水平(P<0.0001)。此外,与饲喂益生菌饮食的鸟类相比,饲喂抗生素饮食的鸟类中SOD的表达降低(P=0.014)。总之,在本研究中测试的肉鸡中鉴定的WB归因于粘蛋白的表达增加,表明WB发生率与凝胶形成粘蛋白分泌和病原体信号传导之间存在相关性。
Previous work has shown that dietary treatments affect woody breast (WB) incidence differently, which indicates that gut conditions such as gut barrier function, inflammation, and oxidative stress are likely related to WB. In this study, dietary supplementation with antibiotics (bacitracin) or probiotics (Bacillus subtilis) was investigated for their effects on the expression of transcripts related to gut barrier function, inflammation, and oxidative stress in the mucus lining of the jejunum from broilers with or without WB. A split-plot experimental design was used in this study. The dietary treatments served as the main plot factor and the breast muscle condition was the subplot factor. On d 41, jejunum mucus was collected from 1 bird from each of 3 replicate pens in each 3 dietary treatment groups that exhibited WB and an additional bird that contained a normal breast (3 biological replicates/treatment/phenotype; 3 × 3 × 2, total N = 18). Total RNA was extracted using a commercial RNA extraction kit. The expression levels of CLDN1, MUC6, TLR2A, TLR2B, TLR4, IFN-γ, IL-1β, IL-8L1, IL-10, NOS2, and SOD were determined using 2-step RT-qPCR analysis. The gene expression difference in ΔCt values was determined after normalizing with the chicken 18S rRNA gene. When the significant differences occurred between treatments, the relative fold change was calculated using the ΔΔCt method and the significance level was calculated. The PROC GLM procedure of SAS 9.4 was used, and the level of significance was set at P ≤ 0.05. There were no significant interactive effects between diet and the breast muscle condition on the expression of any of the genes tested. However, birds with WB exhibited higher MUC6 (P < 0.0001) gene expression levels than birds with normal breast muscles. In addition, the expression of SOD decreased in birds that were fed the antibiotic diet when compared to birds that were fed the probiotic diet (P = 0.014). In conclusion, WB identified in broilers tested in the current study is attributed to increased expression of mucin, indicating a correlation between WB incidence and gel-forming mucin secretion and pathogen signaling.