{Reference Type}: Journal Article
{Title}: Reduced ribonucleotide reductase RRM2 subunit expression increases DNA damage and mitochondria dysfunction in woody breast chickens.
{Author}: Shakeri M;Choi J;Harris C;Buhr RJ;Kong B;Zhuang H;Bowker B;
{Journal}: Am J Vet Res
{Volume}: 85
{Issue}: 4
{Year}: 2024 Apr 1
{Factor}: 1.055
{DOI}: 10.2460/ajvr.23.12.0283
{Abstract}: <B>OBJECTIVE: </B>The aim of this study was to investigate the roles of ribonucleotide reductase subunit M2 (RRM2; subunit of ribonucleotide reductase) in severe woody breast (WB) and normal breast muscles.<BR><B>METHODS: </B>40 8-week-old male Ross-708 broiler chickens.<BR><B>METHODS: </B>Quantitative PCR was performed to determine gene expression, and commercial ELISA/assay kits were used to obtain several enzymatic activities.<BR><B>RESULTS: </B>Results showed that RRM2 activity (P = .0002) and RRM2 (P = .05) and hydroxymethylbilane synthase expression (impaired oxygen transport and metabolism, P = .002) were reduced in WB, while caveolin-3 (defected membrane integrity, P = .09), endoglin (increased fibrosis, P = .06), and secreted protein acidic rich in cysteine (metabolic dysregulation, P = .09) expression tended to increase in WB. WB tended to have increased levels of homocysteine (P = .06), aspartate aminotransferase mitochondria (P = .02), pyruvate kinase (P = .04), DNA damage (P = .06), creatine kinase (P = .05), and triglyceride (P = .002) but decreased ATPase activity (P = .01), all indicating mitochondria dysfunction and tissue damage.<BR><B>CONCLUSIONS: </B>In this study, differences in various enzyme activities and increased DNA damage suggest that RRM2-mediated mitochondrial abnormalities may play a role in WB myopathy.