肠杆菌科细菌耐药性(AMR)的出现和传播对全球公共卫生构成了重大威胁。在这项研究中,我们在泰国南部医院进行了短期监测,以表征基因组多样性,AMR配置文件,肠杆菌科菌株的毒力因子。我们确定了241种耐碳青霉烯的肠杆菌科细菌,其中12个被选择用于全基因组测序(WGS)和基因组分析。这些菌株包括奇异变形杆菌,Nevei沙雷氏菌,Variicola克雷伯菌,产气克雷伯菌,印度克雷伯菌,克雷伯氏菌grimontii,ursingii植物杆菌,棕榈植物细菌,Kosakoniaspp.,和freundii柠檬酸杆菌.这些菌株表现出高水平的多药耐药性,包括对碳青霉烯类抗生素的耐药性.全基因组测序揭示了一系列不同的抗菌素抗性基因(ARGs),带有β-内酰胺酶基因的菌株,外排泵,以及对其他抗生素类别的耐药性。此外,应激反应,金属公差,并鉴定了与毒力相关的基因,突出了这些菌株的适应性和致病潜力。质粒分析确定了几个质粒复制子,包括IncA/C2、IncFIB(K)、和Col440I,以及几个与全球发现的质粒相同的质粒,表明ARGs水平基因转移的潜力。重要的是,这项研究还确定了Kosakoniaspp的一种新物种。PSU27,增加了对泰国南部肠杆菌科的遗传多样性和抗性机制的理解。本研究报告的结果强调了实施有效的抗菌管理计划和开发创新治疗方法以紧急解决AMR的至关重要性。
The emergence and spread of antimicrobial resistance (AMR) among Enterobacteriaceae pose significant threats to global public health. In this study, we conducted a short-term surveillance effort in Southern Thailand hospitals to characterize the genomic diversity, AMR profiles, and virulence factors of Enterobacteriaceae strains. We identified 241 carbapenem-resistant Enterobacteriaceae, of which 12 were selected for whole-genome sequencing (WGS) and genome analysis. The strains included Proteus mirabilis, Serratia nevei, Klebsiella variicola, Klebsiella aerogenes, Klebsiella indica, Klebsiella grimontii, Phytobacter ursingii, Phytobacter palmae, Kosakonia spp., and Citrobacter freundii. The strains exhibited high levels of multidrug resistance, including resistance to carbapenem antibiotics. Whole-genome sequencing revealed a diverse array of antimicrobial resistance genes (ARGs), with strains carrying genes for ß-lactamase, efflux pumps, and resistance to other antibiotic classes. Additionally, stress response, metal tolerance, and virulence-associated genes were identified, highlighting the adaptability and pathogenic potential of these strains. A plasmid analysis identified several plasmid replicons, including IncA/C2, IncFIB(K), and Col440I, as well as several plasmids identical to those found globally, indicating the potential for the horizontal gene transfer of ARGs. Importantly, this study also identified a novel species of Kosakonia spp. PSU27, adding to the understanding of the genetic diversity and resistance mechanisms of Enterobacteriaceae in Southern Thailand. The results reported in this study highlight the critical importance of implementing effective antimicrobial management programs and developing innovative treatment approaches to urgently tackle AMR.