Abstract：
TwoGram-stain-positive and rod-shaped actinomycetes (strains CDC186T and CDC192) were isolated from sputum samples of a patient in Chongqing, PR China, and were investigated to determine their taxonomic status. The results of phylogenetic analysis based on the 16S rRNA gene indicated that CDC186T and CDC192 represented members of the genus Nocardia, and the sequence similarity with Nocardia beijingensis DSM 44636T was the highest, at 99.71 and 99.78 %, respectively. The DNA G+C content of both CDC186T and CDC192 was 69.1 %. Genomic diversity analysis revealed that the average nucleotide identity and in silico DNA‒DNA hybridisation values between the two novel strains and closely related species were significantly below the thresholds of 95-96 and 70 %, respectively, but these values between the two novel strains were 99.96 and 99.90 %, respectively. The phylogenetic relationship based on the dapb1 gene and the single-copy core genes further indicated that the two novel strains were clustered in separate branch adjacent to N. beijingensis DSM 44636T. Growth occurred within the ranges of 20-42 °C, pH 6.0-9.0 and NaCl concentrations of 0.5-4.5 % (w/v). The major fatty acids of CDC186T and CDC192 were C16 : 0 and C18 : 0 10-methyl [tuberculostearic acid (TBSA)]. The predominant respiratory menaquinone was MK-9. The polar lipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside, one unidentified glycolipid, one unidentified phospholipid and one unidentified phosphoglycolipid. All the genomes of the studied strains were annotated with virulence factor (VF)-associated genes homologous to those of Mycobacterium tuberculosis, and the results of susceptibility testing indicated that CDC186T and CDC192 were resistant to amoxicillin-clavulanic acid and tigecycline. On the basis of chemotaxonomic characteristics and the results of phylogenetic analyses, strains CDC186T and CDC192 represent a novel species within the genus Nocardia, for which the name Nocardia implantans sp. nov. is proposed. The type strain is CDC186T (=GDMCC 4.206T= JCM 34959T).
摘要：
从重庆一名患者的痰标本中分离出了二革兰氏染色阳性和杆状放线菌（CDC186T和CDC192菌株），中国公关,并进行了调查以确定它们的分类地位。基于16SrRNA基因的系统发育分析结果表明，CDC186T和CDC192代表诺卡氏菌属的成员，与北京诺卡氏菌DSM44636T的序列相似性最高，在99.71和99.78％，分别。CDC186T和CDC192的DNAG+C含量均为69.1%。基因组多样性分析显示，两个新菌株和密切相关物种之间的平均核苷酸同一性和计算机DNA-DNA杂交值显着低于95-96和70％的阈值，分别,但是这两个新菌株之间的值是99.96％和99.90％，分别。基于dapb1基因和单拷贝核心基因的系统发育关系进一步表明，这两个新菌株聚集在邻近北美洲DSM44636T的单独分支中。生长发生在20-42°C的范围内，pH6.0-9.0，NaCl浓度为0.5-4.5％（w/v）。CDC186T和CDC192的主要脂肪酸为C16：0和C18：010-甲基[结核硬脂酸（TBSA）]。主要的呼吸甲基萘醌是MK-9。极性脂质分布含有二磷脂酰甘油，磷脂酰乙醇胺，磷脂酰肌醇甘露糖苷,一种身份不明的糖脂,一种身份不明的磷脂和一种身份不明的磷酸糖脂。所研究菌株的所有基因组均注释有与结核分枝杆菌同源的毒力因子（VF）相关基因，药敏结果显示CDC186T和CDC192对阿莫西林克拉维酸和替加环素耐药。根据化学分类学特征和系统发育分析的结果，菌株CDC186T和CDC192代表诺卡氏菌属中的一个新物种，对于这个名字，诺卡氏菌植入sp。11月。是提议的。菌株类型为CDC186T(=GDMCC4.206T=JCM34959T)。
