■据报道,阴道菌群与人乳头瘤病毒(HPV)感染有关。目的探讨HPV感染患者宫颈菌群特点,分析HPV感染女性患者阴道菌群和酶谱的变化。
■我们进行了一项横断面研究,涉及206名接受HPV基因分型的参与者,性传播疾病病原体检测,细胞学检查,和微生物组分析。此外,我们收集了115例HPV阴性样本和48例HPV阳性样本进行16SrRNA扩增子测序.分析两组的阴道微生物群落的多样性和差异,以探讨它们与HPV感染的关系。
■发现乳酸菌的丰度降低,而阴道加德纳菌在HPV+组中明显更普遍。就阿尔法多样性指数而言,与HPV-组相比,HPV+组的Shannon指数(P=.0036)和Simpson指数(P=.02)更高,表明HPV+组的群落多样性更大。在分析的10种性传播疾病病原体中,Uup3和Uup6与HPV感染显著相关。两组Nugent评分和细菌性阴道病比较差异有统计学意义(P<0.05)。在功能分析中,在HPV+组中发现11种蛋白质和13种酶显著改变。
■我们的研究表明,阴道菌群的破坏与HPV感染有关。乳酸菌水平降低,加德纳菌的患病率增加,和异常的酶谱与HPV感染密切相关。
本研究的目的是调查人乳头瘤病毒感染患者宫颈菌群的特征,并分析人乳头瘤病毒感染女性患者阴道菌群和酶谱的变化。我们进行了一项横断面研究,涉及206名接受人乳头瘤病毒基因分型的参与者,性传播疾病病原体检测,细胞学检查,和微生物组分析。此外,我们收集了115例HPV阴性样本和48例HPV阳性样本进行16SrRNA扩增子测序.发现乳酸菌的丰度降低,而阴道加德纳菌在HPV+组中明显更普遍。在功能分析中,在HPV+组中发现11种蛋白质和13种酶显著改变。我们的研究表明,阴道菌群的破坏与HPV感染有关。乳酸菌水平降低,加德纳菌的患病率增加,和异常的酶谱与HPV感染密切相关。
UNASSIGNED: The vaginal flora has been reported to be associated with human papillomavirus (HPV) infection. The purpose of this study was to investigate the characteristics of the cervical microbiota in patients with HPV infection and to analyse the changes in the vaginal flora and enzyme profiles in females with HPV infection.
UNASSIGNED: We conducted a cross-sectional study involving 206 participants who underwent HPV genotyping, sexually transmitted diseases pathogen testing, cytology examination, and microbiome analysis. Additionally, we collected 115 HPV-negative samples and 48 HPV-positive samples for 16S rRNA amplicon sequencing. The vaginal microbial communities of both groups were analysed for diversity and differences to explore their association with HPV infection.
UNASSIGNED: The abundance of Lactobacillus was found to be reduced, while Gardnerella vaginalis was significantly more prevalent in the HPV + group. In terms of alpha diversity indices, the Shannon index (P = .0036) and Simpson index (P = .02) were higher in the HPV + group compared to the HPV - group, indicating greater community diversity in the HPV + group. Among the 10 sexually transmitted diseases pathogens analysed, Uup3 and Uup6 were significantly associated with HPV infection. Statistically significant differences were observed in Nugent scores and bacterial vaginosis between the two groups (P < .05). In functional analysis, 11 proteins and 13 enzymes were found to be significantly altered in the HPV + group.
UNASSIGNED: Our study demonstrates that disruptions in the vaginal flora are associated with HPV infection. Reduced levels of Lactobacillus, increased prevalence of Gardnerella, and abnormal enzyme profiles are closely linked to HPV infection.
The purpose of this study was to investigate the characteristics of the cervical microbiota in patients with human papillomavirus infection and to analyse the changes in the vaginal flora and enzyme profiles in females with human papillomavirus infection. We conducted a cross-sectional study involving 206 participants who underwent human papillomavirus genotyping, sexually transmitted diseases pathogen testing, cytology examination, and microbiome analysis. Additionally, we collected 115 HPV-negative samples and 48 HPV-positive samples for 16S rRNA amplicon sequencing. The abundance of Lactobacillus was found to be reduced, while Gardnerella vaginalis was significantly more prevalent in the HPV + group. In functional analysis, 11 proteins and 13 enzymes were found to be significantly altered in the HPV + group. Our study demonstrates that disruptions in the vaginal flora are associated with HPV infection. Reduced levels of Lactobacillus, increased prevalence of Gardnerella, and abnormal enzyme profiles are closely linked to HPV infection.