背景:拟南芥DA1基因是调节器官和种子发育的关键角色。为了扩大我们对水稻功能对应物的理解,这项研究调查了直系同源基因的作用,即DA1,HDR3,HDR3.1和DA2直系同源GW2,通过分析T-DNA插入突变体。
目的:本研究的目的是阐明DA1,HDR3,HDR3.1和GW2中T-DNA插入对水稻农艺性状的影响。通过评估纯合植物,我们特别关注关键参数,如植物高度,舵柄编号,天的标题,和晶粒尺寸。
方法:使用PCR验证T-DNA插入位置,和随后的分析在纯合植物上进行。农艺性状,包括植物高度,舵柄编号,天的标题,和晶粒尺寸,被评估。此外,在黑暗孵育条件下进行叶片衰老测定以测量T-DNA插入对该生理方面的影响。
结果:该研究揭示了与HDR3、HDR3.1、GW2和DA1中T-DNA插入相关的独特表型结果。具体来说,HDR3和HDR3.1突变体表现出显著降低的株高和较小的晶粒尺寸,与野生型品种东金相比,GW2和DA1突变体的株高和籽粒大小均显着增加。叶片衰老测定进一步表明hdr3.1突变体的叶片衰老延迟,与在黑暗孵育下在hdr3突变体中观察到的稍早的叶片衰老形成对比。
结论:研究结果强调了DA1直系同源基因在水稻中的关键作用,阐明它们在调节植物生长发育中的意义。观察到的表型变异突出了这些基因作为作物改良策略目标的潜力,提供有助于提高水稻和潜在其他作物农艺性状的见解。
The Arabidopsis DA1 gene is a key player in the regulation of organ and seed development. To extend our understanding of its functional counterparts in rice, this study investigates the roles of orthologous genes, namely DA1, HDR3, HDR3.1, and the DA2 ortholog GW2, through the analysis of T-DNA insertion mutants.
The aim of this research is to elucidate the impact of T-DNA insertions in DA1, HDR3, HDR3.1, and GW2 on agronomic traits in rice. By evaluating homozygous plants, we specifically focus on key parameters such as plant height, tiller number, days to heading, and grain size.
T-DNA insertion locations were validated using PCR, and subsequent analyses were conducted on homozygous plants. Agronomic traits, including plant height, tiller number, days to heading, and grain size, were assessed. Additionally, leaf senescence assays were performed under dark incubation conditions to gauge the impact of T-DNA insertions on this physiological aspect.
The study revealed distinctive phenotypic outcomes associated with T-DNA insertions in HDR3, HDR3.1, GW2, and DA1. Specifically, HDR3 and HDR3.1 mutants exhibited significantly reduced plant height and smaller grain size, while GW2 and DA1 mutants displayed a notable increase in both plant height and grain size compared to the wild type variety Dongjin. Leaf senescence assays further indicated delayed leaf senescence in hdr3.1 mutants, contrasting with slightly earlier leaf senescence observed in hdr3 mutants under dark incubation.
The findings underscore the pivotal roles of DA1 orthologous genes in rice, shedding light on their significance in regulating plant growth and development. The observed phenotypic variations highlight the potential of these genes as targets for crop improvement strategies, offering insights that could contribute to the enhancement of agronomic traits in rice and potentially other crops.