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Abstract：
This review explores various methods for modulating protein stability to achieve target protein degradation, which is a crucial aspect in the study of biological processes and drug design. Thirty years have passed since the introduction of heat-inducible degron cells utilizing the N-end rule, and methods for controlling protein stability using the ubiquitin-proteasome system have moved from academia to industry. This review covers protein stability control methods, from the early days to recent advancements, and discusses the evolution of techniques in this field. This review also addresses the challenges and future directions of protein stability control techniques by tracing their development from the inception of protein stability control methods to the present day.
摘要：
本文探讨了调节蛋白质稳定性以实现靶蛋白降解的各种方法,这是研究生物过程和药物设计的一个重要方面。自利用N端规则引入热诱导的Degron细胞以来，已经过去了30年，以及使用泛素-蛋白酶体系统控制蛋白质稳定性的方法已经从学术界转移到了工业界。这篇综述涵盖了蛋白质稳定性控制方法，从早期到最近的进步，并讨论了该领域技术的发展。这篇综述还通过追踪从蛋白质稳定性控制方法开始到现在的发展，解决了蛋白质稳定性控制技术的挑战和未来方向。
