最近的研究揭示了miRNA在甲状腺乳头状癌(PTC)的诊断和临床演变中的重要性。我们旨在鉴定可以区分PTC的特定亚型的特定miRNA谱。
方法:在这项横断面研究中,从43例患者的石蜡包埋组织中提取总RNA,17具有PTC的浸润性滤泡变体(iFVPTC)和26具有PTC的常规变体(cPTC)。使用qRT-PCR技术和特异性miRNA测定评估了9种miRNA。
结果:我们发现了cPTC和iFVPTC的特定模式,例如在两种类型的肿瘤中改变的miRNA(miR-146b-5p,miR-181a-5p,miR-221-3p,miR-21-5p和miR-222-3p)和两个仅在cPTC中显著表达的miRNA(miR-20b-5p,miR-21-5p)。iFVPTC组呈现miRNA表达与临床数据之间的强和中等相关性。miR-221-3p,miR-195-5p,miR-181-5p,miR-146b-5p和miR-222与年龄相关,肿瘤大小(TS)或淋巴结转移(N),而只有miR-20b-5p,miR-195-5p和miR-181-5p与TS相关,cPTC组的N和年龄。
结论:本研究允许鉴定两种miRNA的特征以确认TC的两种组织学亚型之间的miRNA差异。我们的结果提供了TC分子诊断的进展,并可能有助于提高现有分子分类器的诊断性能。
Recent research has revealed the importance of miRNAs in the diagnosis and clinical evolution of papillary thyroid cancer (PTC). We aim to identify a specific miRNA profile that could differentiate between specific subtypes of PTC.
METHODS: In this cross-sectional study, total RNA was extracted from paraffin-embedded tissues of 43 patients, 17 with an infiltrative follicular variant of PTC (iFVPTC) and 26 with a conventional variant of PTC (cPTC). Nine miRNAs were evaluated using qRT-PCR technology and specific miRNA assays.
RESULTS: We found specific patterns for cPTC and iFVPTC, such as miRNA altered in both types of tumours (miR-146b-5p, miR-181a-5p, miR-221-3p, miR-21-5p and miR-222-3p) and two miRNAs significantly expressed only in cPTC (miR-20b-5p, miR-21-5p). The iFVPTC group presented strong and moderate correlations between miRNA expression and clinical data. miR-221-3p, miR-195-5p, miR-181-5p, miR-146b-5p and miR-222 were correlated with age, tumour size (TS) or lymph node metastases (N), while only miR-20b-5p, miR-195-5p and miR-181-5p were correlated with TS, N and age in the cPTC group.
CONCLUSIONS: The present study allowed the identification of a signature of two miRNAs to confirm miRNA differences between the two histological subtypes of TC. Our results provide advances in the molecular diagnosis of TC and could help to improve the diagnostic performance of already existing molecular classifiers.