Utricularia(bladderworts)属是食肉植物,它们使用具有高速抽吸机制的诱捕器捕食无脊椎动物。外部陷阱表面衬有圆顶状的腺体,负责在活动陷阱中分泌水。在这些腺体的末端细胞中,外墙分为几层,甚至细胞壁向内生长也被新的细胞壁层覆盖。由于细胞壁的变化,这些腺体是研究细胞壁(微域)特化的极好模型。这项研究的主要目的是检查不同的细胞壁层是否具有不同的组成。使用抗阿拉伯半乳聚糖蛋白(AGP)的抗体,包括JIM8、JIM13、JIM14、MAC207和JIM4。使用免疫组织化学技术和免疫金标记确定所检查化合物的定位。在终末腺细胞中,原代细胞壁和细胞次生壁之间存在组成差异。终端电池的细胞壁的最外层,它是网状的,没有AGP(JIM8,JIM14)。相比之下,终末细胞的次生细胞壁富含AGP。与JIM13,JIM8和JIM14表位定位的AGP发生在基座细胞的壁向内生长中。我们的研究支持外腺分泌水的假设。
The genus Utricularia (bladderworts) species are carnivorous plants that prey on invertebrates using traps with a high-speed suction mechanism. The outer trap surface is lined by dome-shaped glands responsible for secreting water in active traps. In terminal cells of these glands, the outer wall is differentiated into several layers, and even cell wall ingrowths are covered by new cell wall layers. Due to changes in the cell wall, these glands are excellent models for studying the specialization of cell walls (microdomains). The main aim of this study was to check if different cell wall layers have a different composition. Antibodies against arabinogalactan proteins (AGPs) were used, including JIM8, JIM13, JIM14, MAC207, and JIM4. The localization of the examined compounds was determined using immunohistochemistry techniques and immunogold labeling. Differences in composition were found between the primary cell wall and the cell secondary wall in terminal gland cells. The outermost layer of the cell wall of the terminal cell, which was cuticularized, was devoid of AGPs (JIM8, JIM14). In contrast, the secondary cell wall in terminal cells was rich in AGPs. AGPs localized with the JIM13, JIM8, and JIM14 epitopes occurred in wall ingrowths of pedestal cells. Our research supports the hypothesis of water secretion by the external glands.