背景:茶树(互叶千层)以生产茶树油而闻名,一种精油,主要由从枝条中提取的萜烯组成。MYB转录因子,是最大的TF家族之一,在调节初级和次级代谢产物的合成中起着至关重要的作用。然而,对互叶互叶分枝杆菌MYB基因家族的了解有限。
结果:这里,我们对互花分枝杆菌中的MYB基因进行了全面的全基因组分析,被称为MaMYB,包括系统发育关系,结构,启动子区域,和GO注释。我们的发现将219个MaMYB分为四个亚家族:一个5R-MYB,四个3R-MYB,61个MYB相关,剩下的153个都是2R-MYB.基于GO注释,七个基因(MYB189、MYB146、MYB44、MYB29、MYB175、MYB162和MYB160)与萜类化合物合成相关。拟南芥同源MYB基因的系统发育分析表明,MYB193和MYB163也可能参与萜类化合物的合成。此外,通过基因表达和代谢物含量的相关性分析,我们鉴定了42个与代谢物含量相关的MYB基因。
结论:这些结果提供了有关MYB转录因子在互叶菊精油生产中的重要性的有价值的见解。这些发现为更好地理解MYB调控网络和开发新策略以增强互花菊精油合成奠定了基础。
BACKGROUND: The tea tree (Melaleuca alternifolia) is renowned for its production of tea tree oil, an essential oil primarily composed of terpenes extracted from its shoot. MYB transcription factors, which are one of the largest TF families, play a crucial role in regulating primary and secondary metabolite synthesis. However, knowledge of the MYB gene family in M. alternifolia is limited.
RESULTS: Here, we conducted a comprehensive genome-wide analysis of MYB genes in M. alternifolia, referred to as MaMYBs, including phylogenetic relationships, structures, promoter regions, and GO annotations. Our findings classified 219 MaMYBs into four subfamilies: one 5R-MYB, four 3R-MYBs, sixty-one MYB-related, and the remaining 153 are all 2R-MYBs. Seven genes (MYB189, MYB146, MYB44, MYB29, MYB175, MYB162, and MYB160) were linked to terpenoid synthesis based on GO annotation. Phylogenetic analysis with Arabidopsis homologous MYB genes suggested that MYB193 and MYB163 may also be involved in terpenoid synthesis. Additionally, through correlation analysis of gene expression and metabolite content, we identified 42 MYB genes associated with metabolite content.
CONCLUSIONS: The results provide valuable insights into the importance of MYB transcription factors in essential oil production in M. alternifolia. These findings lay the groundwork for a better understanding of the MYB regulatory network and the development of novel strategies to enhance essential oil synthesis in M. alternifolia.