罗望子壳富含黄酮类化合物,具有良好的生物活性。在这项研究中,我们旨在分析罗望子壳提取物(TSE)的化学成分,并研究TSE的体内外抗氧化能力。罗望子壳用95%乙醇回流提取,采用超高效液相色谱-电喷雾串联质谱(UPLC-MS/MS)测定化学成分。使用氧自由基吸收能力(ORAC)方法评估TSE的体外自由基清除活性。在2,2-偶氮双(2-脒基丙烷)二盐酸盐(AAPH)刺激的ADTC5细胞和暴露于叔丁基过氧化氢(t-BHP)的斑马鱼中进一步评估了TSE的抗氧化作用。在TSE中总共检测到8种黄酮类化合物,包括(+)-儿茶素,taxifolin,杨梅素,安的酚,木犀草素,Morin,芹菜素,还有柚皮素,含量分别为5.287、8.419、4.042、6.583、3.421、4.651、0.2027和0.6234mg/g,分别。ORAC分析显示TSE和这些类黄酮在体外具有很强的自由基清除活性。此外,TSE显着降低了AAPH处理的ATDC5细胞和暴露于t-BHP的斑马鱼的ROS和MDA水平,但恢复了SOD活性。黄酮类化合物还对ATDC5细胞和斑马鱼的氧化损伤表现出优异的抗氧化活性。总的来说,本研究提示了TSE及其主要黄酮在体内外的自由基清除能力和抗氧化能力,为罗望子壳的开发利用提供了理论依据。
Tamarind shell is rich in flavonoids and exhibits good biological activities. In this study, we aimed to analyze the chemical composition of tamarind shell extract (TSE), and to investigate antioxidant capacity of TSE in vitro and in vivo. The tamarind shells were extracted with 95% ethanol refluxing extraction, and chemical constituents were determined by ultra-performance chromatography-electrospray tandem mass spectrometry (UPLC-MS/MS). The free radical scavenging activity of TSE in vitro was evaluated using the oxygen radical absorbance capacity (ORAC) method. The antioxidative effects of TSE were further assessed in 2,2-azobis (2-amidinopropane) dihydrochloride (AAPH)-stimulated ADTC5 cells and tert-butyl hydroperoxide (t-BHP)-exposed zebrafish. A total of eight flavonoids were detected in TSE, including (+)-catechin, taxifolin, myricetin, eriodictyol, luteolin, morin, apigenin, and naringenin, with the contents of 5.287, 8.419, 4.042, 6.583, 3.421, 4.651, 0.2027, and 0.6234 mg/g, respectively. The ORAC assay revealed TSE and these flavonoids had strong free radical scavenging activity in vitro. In addition, TSE significantly decreased the ROS and MDA levels but restored the SOD activity in AAPH-treated ATDC5 cells and t-BHP-exposed zebrafish. The flavonoids also showed excellent antioxidative activities against oxidative damage in ATDC5 cells and zebrafish. Overall, the study suggests the free radical scavenging capacity and antioxidant potential of TSE and its primary flavonoids in vitro and in vivo and will provide a theoretical basis for the development and utilization of tamarind shell.