PDF(Pubmed)
Abstract：
There are different estimates for the incidence of infertility. Its occurrence may vary from area to area, but on average, it affects 15% of couples and 10-12% of men worldwide. Many aspects of infertility can be linked to reactive oxygen species (ROS) and the process of oxidative stress (OS). The association between poor semen quality and OS is well known. Unfortunately, there is no accepted protocol for the diagnosis and treatment of OS in andrology. Oxido-reduction potential (ORP) measurement is a new method for determining the ratio between oxidant and antioxidant molecules. Currently, ORP measurement is one of the fastest and most user-friendly methods of andrological OS determination and our goals were to confirm published correlations between ORP values and sperm parameters, examine how sperm concentration influences these results, and investigate whether intracellular ROS formations are also manifested in the ORP values or not after artificial ROS induction. Intracellular ROS formations were induced by menadione (superoxide anion inducer), hydrogen peroxide, and tert-butyl hydroperoxide (lipid peroxidation inducer) treatments; sperm parameters like motility and viability were determined with an SCA Scope system, and ORP changes were recorded by the Mioxsys system. Significant correlations were noticed among the ORP, spermatozoa concentration, motility, progressive motility, and viability. Nevertheless, only the ORP value after normalization with the sperm count correlated with these parameters. Due to normalization, very low and very high sperm concentrations can give misleading results. The means of the non-normalized ORP values were almost the same. All of the applied treatments resulted in decreases in the viability, motility, and progressive motility, and interestingly, altered ORP levels were detected. In addition, it was determined that seminal plasma had a significant protective effect on spermatozoa. The elimination of seminal plasma caused higher sensitivity of spermatozoa against used OS inducers, and higher ORP levels and decreased viabilities and motilities were measured. The ORP level could be a good indicator of male OS; however, in cases of low and high sperm counts, its result can be misleading. Overall, the conclusion can be drawn that ORP determination is a suitable method for detecting intracellular ROS accumulation, but it has limitations that still need to be clarified.
摘要：
对不孕症的发病率有不同的估计。它的发生可能因地区而异，但平均而言,它影响了全世界15%的夫妇和10%-12%的男性。不育的许多方面可以与活性氧（ROS）和氧化应激（OS）的过程有关。精液质量差与OS之间的关联是众所周知的。不幸的是,对于男科OS的诊断和治疗,目前尚无公认的方案.氧化还原电位（ORP）测量是确定氧化剂和抗氧化剂分子之间比例的一种新方法。目前,ORP测量是男性OS确定的最快和最用户友好的方法之一，我们的目标是确认已发表的ORP值和精子参数之间的相关性，检查精子浓度如何影响这些结果，并研究人工诱导ROS后，细胞内ROS的形成是否也表现在ORP值中。细胞内ROS形成由甲萘醌（超氧阴离子诱导剂）诱导，过氧化氢,和叔丁基过氧化氢（脂质过氧化诱导剂）处理；使用SCAScope系统确定精子参数，如运动能力和活力，和ORP变化由Mioxsys系统记录。注意到ORP之间存在显着相关性，精子浓度，运动性,渐进运动，和生存能力。然而，只有与精子计数归一化后的ORP值与这些参数相关。由于正常化，非常低和非常高的精子浓度会产生误导性的结果。非归一化ORP值的平均值几乎相同。所有应用的处理都导致生存力下降，运动性,和渐进的运动，有趣的是，检测到ORP水平改变。此外,确定精浆对精子有明显的保护作用。精浆的消除导致精子对使用的OS诱导剂的敏感性更高，并测量了较高的ORP水平以及降低的活力和运动能力。ORP水平可能是男性OS的良好指标；然而，在精子数量低和高的情况下，其结果可能具有误导性。总的来说,可以得出结论，ORP测定是检测细胞内ROS积累的合适方法，但是它有局限性，仍然需要澄清。
