已经证明内分泌干扰物通过破坏激素水平对两栖动物的生长和发育产生不利影响。尾部再吸收,这是两栖动物变态中最引人注目的事件之一,与甲状腺激素水平密切相关。然而,关于内分泌干扰物对两栖动物尾巴吸收的影响的研究有限。本研究探索了NaClO4和T4对生长的影响,中国林蛙变态过程中的发育和尾部吸收。结果表明,暴露于NaClO4会导致R.Chensinensist的体型增加和变态延迟。组织学分析显示,NaClO4和外源性T4暴露导致甲状腺损伤,NaClO4治疗延缓了脊索和肌肉的退化,从而延迟尾部再吸收。此外,转录组测序结果显示,凋亡相关基因(APAF1、BAX和CASP6)和细胞成分降解相关基因(MMP9和MMP13)在T4暴露组中高表达,NaClO4暴露组氧化应激相关基因(SOD和CAT)表达较高。一起来看,NaClO4和外源T4都会影响中国红的尾部吸收,从而影响他们对陆地生活的适应。本研究不仅为今后其他内分泌干扰物对其生长影响的实验研究提供参考,两栖动物的发展和尾部吸收,也将为环境保护和生态风险评估提供见解。
Endocrine disruptors have been demonstrated to exert adverse effects on growth and development of amphibians by disrupting hormone levels. Tail resorption, which is one of the most remarkable events during amphibian metamorphosis, is closely associated with thyroid hormones levels. However, limited research has been conducted on the effects of endocrine disruptors on tail resorption in amphibians. This study explored the effects of NaClO4 and T4 on the growth, development and tail resorption during the metamorphosis of Rana Chensinensis. The results demonstrated that exposure to NaClO4 led to an increase in body size and a delay in metamorphosis of R. Chensinensis tadpoles. Histological analysis revealed that both NaClO4 and exogenous T4 exposure resulted in thyroid gland injury, and NaClO4 treatment delayed the degradation of notochord and muscles, thereby delaying tail resorption. Moreover, transcriptome sequencing results showed that apoptosis-related genes (APAF1, BAX and CASP6) and cell component degradation-related genes (MMP9 and MMP13) were highly expressed in the T4 exposure group, and the expression of oxidative stress-related genes (SOD and CAT) was higher in the NaClO4 exposure group. Taken together, both NaClO4 and exogenous T4 affect tail resorption in R. Chensinensis, thereby affecting their adaptation to terrestrial life. The present study will not only provide a reference for future experimental research on the effects of other endocrine disruptors on the growth, development and tail resorption of amphibians but will also provide insights into environmental protection and ecological risk assessment.