背景:本研究旨在对足月孕妇的口腔微生物群与母亲的局部胎盘免疫和全身免疫系统之间的相关性进行初步探索。
方法:共有26名孕妇参加了这项研究,从口腔拭子收集的样本,胎盘组织,和外周静脉血。高通量测序用于检查口腔微生物群落。采用流式细胞术评估胎盘组织和外周静脉血中的免疫细胞。采用ELISA和Luminex液珠芯片技术检测胎盘组织和外周静脉血中的细胞因子。
结果:在胎盘组织中,口腔微生物群落主要与胎盘CD3+CD4+CD8+T细胞呈负相关,与胎盘IL-5呈正相关。在外周血中,口腔微生物群落主要与母体全身免疫参数呈正相关,包括CD3+CD4+T细胞和CD4+/CD8+比值,以及与外周IL-18呈正相关。
结论:足月孕妇口腔菌群参与母体免疫系统的调节功能。同时,口腔微生物群落也可能是介导胎盘局部免疫调节的重要因素。
BACKGROUND: This study aims to conduct a preliminary exploration of the correlation between the oral microbiota of full-term pregnant women and both local placental immunity and the systemic immune system of the mother.
METHODS: A total of 26 pregnant women participated in this study, with samples collected from oral swabs, placental tissue, and peripheral venous blood. High-throughput sequencing was used to examine the oral microbial community. Flow cytometry was employed to assess immune cells in placental tissue and peripheral venous blood. ELISA and Luminex liquid bead chip technology were utilized to detect cytokines in both placental tissue and peripheral venous blood.
RESULTS: In placental tissue, The oral microbial community is primarily negatively correlated with placental CD3+CD4+CD8+T cells and positively correlated with placental IL-5. In the peripheral blood, The oral microbial community is primarily positively correlated with maternal systemic immune parameters, including CD3+CD4+ T cells and the CD4+/CD8+ ratio, as well as positively correlated with peripheral IL-18.
CONCLUSIONS: The oral microbiota of full-term pregnant women participates in the regulatory function of the maternal immune system. Meanwhile, the oral microbial community may also be an important factor mediating local immune regulation in the placenta.