{Reference Type}: Journal Article {Title}: Spatiotemporal single-cell analysis decodes cellular dynamics underlying different responses to immunotherapy in colorectal cancer. {Author}: Chen Y;Wang D;Li Y;Qi L;Si W;Bo Y;Chen X;Ye Z;Fan H;Liu B;Liu C;Zhang L;Zhang X;Li Z;Zhu L;Wu A;Zhang Z; {Journal}: Cancer Cell {Volume}: 42 {Issue}: 7 {Year}: 2024 Jul 8 {Factor}: 38.585 {DOI}: 10.1016/j.ccell.2024.06.009 {Abstract}: Expanding the efficacy of immune checkpoint blockade (ICB) in colorectal cancer (CRC) presses for a comprehensive understanding of treatment responsiveness. Here, we analyze multiple sequential single-cell samples from 22 patients undergoing PD-1 blockade to map the evolution of local and systemic immunity of CRC patients. In tumors, we identify coordinated cellular programs exhibiting distinct response associations. Specifically, exhausted T (Tex) or tumor-reactive-like CD8+ T (Ttr-like) cells are closely related to treatment efficacy, and Tex cells show correlated proportion changes with multiple other tumor-enriched cell types following PD-1 blockade. In addition, we reveal the less-exhausted phenotype of blood-associated Ttr-like cells in tumors and find that their higher abundance suggests better treatment outcomes. Finally, a higher major histocompatibility complex (MHC) II-related signature in circulating CD8+ T cells at baseline is linked to superior responses. Our study provides insights into the spatiotemporal cellular dynamics following neoadjuvant PD-1 blockade in CRC.