目的:引起肠道疾病的病原微生物可显著危害人类健康。目前,没有授权的治疗或疫苗接种来对抗导致肠道疾病的细菌。
方法:使用免疫信息学,我们开发了一种有效的多表位组合(组合)疫苗对抗沙门氏菌和肠出血性大肠杆菌。B和T细胞表位通过进行保护性评估来鉴定,人口覆盖率分析,物理化学属性评估,以及所选择的抗原多肽的二级和三级结构评估。疫苗开发的选择过程包括使用几种生物信息学工具和方法最终选择两个线性B细胞表位,5个CTL表位,和两个HTL表位。
结果:该疫苗具有很强的免疫原性,细胞因子产生,免疫学特性,无毒性,非过敏性,稳定性,以及对感染的潜在功效。二硫化物粘结,密码子修饰,和计算克隆也用于增强在宿主大肠杆菌中表达的稳定性和功效。该疫苗的结构对TLR4配体具有很强的亲和力,并且非常耐用,如分子对接和分子建模所示。免疫学模拟的结果表明B细胞和T细胞都对疫苗接种组分具有增强的应答。
结论:全面的计算机分析显示,所提出的疫苗可能会引发针对引起肠道疾病的病原菌的强大免疫反应。因此,对于进一步的实验测试,这是一个很有希望的选择。
OBJECTIVE: The pathogenic microorganisms that cause intestinal diseases can significantly jeopardize people\'s health. Currently, there are no authorized treatments or vaccinations available to combat the germs responsible for intestinal disease.
METHODS: Using immunoinformatics, we developed a potent multi-epitope Combination (combo) vaccine versus Salmonella and enterohemorrhagic E. coli. The B and T cell epitopes were identified by performing a conservancy assessment, population coverage analysis, physicochemical attributes assessment, and secondary and tertiary structure assessment of the chosen antigenic polypeptide. The selection process for vaccine development included using several bioinformatics tools and approaches to finally choose two linear B-cell epitopes, five CTL epitopes, and two HTL epitopes.
RESULTS: The vaccine had strong immunogenicity, cytokine production, immunological properties, non-toxicity, non-allergenicity, stability, and potential efficacy against infections. Disulfide bonding, codon modification, and computational cloning were also used to enhance the stability and efficacy of expression in the host E. coli. The vaccine\'s structure has a strong affinity for the TLR4 ligand and is very durable, as shown by molecular docking and molecular modeling. The results of the immunological simulation demonstrated that both B and T cells had a heightened response to the vaccination component.
CONCLUSIONS: The comprehensive in silico analysis reveals that the proposed vaccine will likely elicit a robust immune response against pathogenic bacteria that cause intestinal diseases. Therefore, it is a promising option for further experimental testing.