■面对人口的增加和资源的减少,食品安全是一个严峻的挑战。金黄色葡萄球菌是一种重要的食源性病原体,其特征是能够分泌各种耐热肠毒素。在牛群中使用抗生素导致细菌种类之间发生抗菌素耐药性(AMR)模式,因此通过乳制品传播给人类。乳酸菌(LAB)产生细菌素,它提供了天然抗菌剂的极好来源,并具有环保和安全的进一步优势。
■检测相关样品中的多药耐药性(MDR)金黄色葡萄球菌分离株,分子特征,生物膜生产,以及LAB对它的抑制作用。
■对原料乳和其他乳制品的随机样品进行金黄色葡萄球菌分离分析。进行AMR的表型和基因型评估,除了检测金黄色葡萄球菌的经典肠毒素基因。最后,一些乳酸菌菌株对金黄色葡萄球菌的抗菌作用的评估。
■原奶中推定金黄色葡萄球菌的发病率,Kariesh奶酪,酸奶样本占50%,40%,60%,分别。金黄色葡萄球菌对卡那霉素(100%)和萘啶酸(89.3%)的耐药性最高,分别。金黄色葡萄球菌(78.66%)为MDR。11.1%的金黄色葡萄球菌携带mecA基因。关于肠毒素基因,PCR显示,被检查的分离株具有海洋的百分比(22.2%),而sed在(11.1%)的分离株中被发现。关于生物膜的生产,(88.88%)的金黄色葡萄球菌是生物膜生产者。最后,琼脂扩散表明,嗜酸乳杆菌对金黄色葡萄球菌的抑菌效果最强,抑菌圈直径为18~22mm。
■MDR金黄色葡萄球菌在原料乳和乳制品中普遍存在。葡萄球菌肠毒素的生产,以及生物膜生产是造成公共卫生风险的原因。因此,在食物链层面安装适当的卫生程序和严格的食品安全政策是很重要的。
UNASSIGNED: Food safety is a serious challenge in the face of increasing population and diminishing resources. Staphylococcus aureus is a critical foodborne pathogen characterized by its capability to secret a diverse range of heat-resistant enterotoxins. Antibiotic usage in dairy herds resulted in the occurrence of antimicrobial resistance (AMR) patterns among bacterial species, which were consequently transmitted to humans via dairy products. Lactic acid bacteria (LAB) produce bacteriocins, which provide an excellent source of natural antimicrobials with the further advantage of being environmentally friendly and safe.
UNASSIGNED: Detection of multidrug resistance (MDR) S. aureus isolates in concerned samples, molecular characteristics, biofilm production, and the inhibitory role of LAB against it.
UNASSIGNED: Random samples of raw milk and other dairy products were analyzed for S. aureus isolation. Phenotypic and genotypic assessment of AMR was performed, in addition to detection of classical enterotoxin genes of S. aureus. Finally, evaluation of the antimicrobial action of some Lactobacillus strains against S. aureus.
UNASSIGNED: Incidence rates of presumptive S. aureus in raw milk, Kariesh cheese, and yogurt samples were 50%, 40%, and 60%, respectively. The highest resistance of S. aureus was to Kanamycin (100%) and Nalidixic acid (89.3%), respectively. (78.66%) of S. aureus were MDR. 11.1% of S. aureus carried mecA gene. In concern with enterotoxins genes, PCR showed that examined isolates harbored sea with a percentage of (22.2%), while sed was found in (11.1%) of isolates. Regarding biofilm production, (88.88%) of S. aureus were biofilm producers. Finally, agar well diffusion showed that Lactobacillus acidophilus had the strongest antimicrobial action against S. aureus with inhibition zone diameter ranging from 18 to 22 mm.
UNASSIGNED: There is a widespread prevalence of MDR S. aureus in raw milk and dairy products. Production of staphylococcal enterotoxins, as well as biofilm production are responsible for public health risks. Therefore, installing proper hygienic routines and harsh food safety policies at food chain levels is substantial.