PDF(Pubmed)
Abstract：
Some rodent species cause significant damage to agriculture and forestry, and some can transmit pathogens to humans and livestock. The common vole (Microtus arvalis) is widespread in Europe, and its population outbreaks have resulted in massive crop loss. Bait-based fertility control could contribute to rodent pest management. Bait containing 4-vinylcyclohexene diepoxide (VCD) and triptolide (TP), registered as ContraPest®, was delivered to male common voles for 14 or 28 consecutive days. The effects on reproductive structures and residues in the liver and testes were assessed. There was no effect on testis weight, sperm viability, sperm motility and oxidative stress in sperm cells. Results regarding the mitochondrial membrane potential of sperm, DNA fragmentation and progressively motile sperm cells were inconclusive. However, there was an increase in morphological sperm defects in voles treated for 14/28 days and fewer normal sperm cells in voles treated for 28 days. There were no TP residues in the testes, few and low TP residues and no VCD residues in liver tissues, making considerable secondary exposure to non-target species unlikely. Treatments with VCD + TP seemed to have minor effects on the reproductive organs of males. Further studies should evaluate the effect of VCD + TP on females and on the reproductive success of common voles and other pest rodent species.
摘要：
一些啮齿动物物种对农业和林业造成重大损害，有些可以将病原体传播给人类和牲畜。普通田鼠（Microtusarvalis）在欧洲很普遍，其人口爆发导致了大规模的作物损失。基于诱饵的生育力控制可能有助于啮齿动物害虫的管理。含4-乙烯基环己烯二环氧化物（VCD）和雷公藤甲素（TP）的诱饵，注册为ContraPest®，连续14或28天交付给雄性普通田鼠。评估了对肝脏和睾丸中生殖结构和残留物的影响。对睾丸重量没有影响,精子活力，精子运动和精子细胞的氧化应激。关于精子线粒体膜电位的结果，DNA片段化和逐渐活动的精子细胞尚无定论。然而,治疗14/28天的田鼠精子形态缺陷增加,治疗28天的田鼠正常精子细胞减少.睾丸中没有TP残留,肝脏组织中TP残基少且低，无VCD残基，使得相当多的二次暴露于非目标物种的可能性不大。用VCD+TP治疗似乎对男性的生殖器官影响较小。进一步的研究应评估VCDTP对雌性以及普通田鼠和其他害虫啮齿动物繁殖成功的影响。
