目的:这项研究开发了溶胶-凝胶硅酸三钙/氧化石墨烯(TCS-GO)复合材料,并检查了其理化性质,抗菌活性,和对牙髓干细胞的骨/牙源性作用。
方法:合成了硅酸三钙,并在三种不同浓度下与氧化石墨烯结合,即0.02%,0.04%,和0.08%w/w,而硅酸三钙和矿物三氧化物聚集体作为对照。凝固时间,抗压强度,pH值,并对复合材料的钙离子释放进行了评估,以及对变形链球菌和嗜酸乳杆菌的抗菌特性。此外,牙髓干细胞活力;磷灰石形成能力;碱性磷酸酶基因表达,牙本质唾液磷蛋白,和Runt相关转录因子2进行评估。
结果:与MTA相比,TCS-GO(0.08%)显示出明显更短的凝固时间和更高的抗压强度(p<0.05)。此外,硅酸三钙和TCS-GO组显示出比MTA更高的Ca离子释放,不同组间pH值无显著差异。与MTA相比,TCS-GO(0.08%)还表现出对嗜酸乳杆菌的显著更强的抗微生物作用(p<0.05)。在第3天和第7天,TCS-GO中的ALP表达(0.08%)高于MTA,而在第3天,TCS-GO中的DSPP表达(0.08%)高于MTA,但在第7天逆转。在第3天和第7天,TCS-GO(0.08%)和MTA之间的RUNX2表达没有显著差异。
结论:与MTA相比,TCS-GO(0.08%)复合材料表现出优异的理化特性和抗菌性能。此外,TCS-GO中ALP和DSPP标记物的早期上调(0.08%)表明它具有促进和增强DPSCs的骨/牙源性分化的潜力。
OBJECTIVE: This study developed a sol-gel tricalcium silicate/graphene oxide (TCS-GO) composite and examined its physicochemical properties, antimicrobial activity, and osteo/odontogenic effect on dental pulp stem cells.
METHODS: Tricalcium silicate was synthesized and combined with graphene oxide at three different concentrations, namely 0.02%, 0.04%, and 0.08% w/w, while tricalcium silicate and mineral trioxide aggregate served as controls. The setting time, compressive strength, pH, and calcium ion release of the composites were evaluated, as well as antimicrobial properties against Streptococcus mutans and Lactobacillus acidophilus. Additionally, the viability of dental pulp stem cells; apatite forming ability; and the gene expression of Alkaline phosphatase, Dentin sialophosphoprotein, and Runt-related transcription factor 2 were assessed.
RESULTS: TCS-GO (0.08%) showed a significantly shorter setting time and higher compressive strength when compared to MTA (p < 0.05). Additionally, tricalcium silicate and TCS-GO groups showed a higher release of Ca ions than MTA, with no significant difference in pH values among the different groups. TCS-GO (0.08%) also demonstrated a significantly stronger antimicrobial effect against Lactobacillus acidophilus compared to MTA (p < 0.05). ALP expression was higher in TCS-GO (0.08%) than MTA on days 3 and 7, while DSPP expression was higher in TCS-GO (0.08%) than MTA on day 3 but reversed on day 7. There was no significant difference in RUNX2 expression between TCS-GO (0.08%) and MTA on days 3 and 7.
CONCLUSIONS: The TCS-GO (0.08%) composite demonstrated superior physicochemical characteristics and antimicrobial properties compared to MTA. Moreover, the early upregulation of ALP and DSPP markers in TCS-GO (0.08%) indicates that it has the potential to promote and enhance the osteo/odontogenic differentiation of DPSCs.