■线粒体自噬和铁凋亡发生在脑出血(ICH)中,但我们对线粒体自噬和铁凋亡相关基因的理解仍然不完整。
■本研究旨在确定两种过程的共有ICH基因。
■ICH差异表达的线粒体自噬和铁凋亡相关基因(DEMFRGs)来源于GEO数据库和文献。富集分析阐明了功能。Hub基因是通过STRING选择的,MCODE,和Cytoscape中的MCC算法。使用miRWalk3.0预测miRNA靶向中心,形成miRNA-hub基因网络。用MCP和TIMER评估免疫微环境变化。通过CMap数据库预测ICH的潜在小分子。
■64个DEMFRGs和10个hub基因可能参与各种过程,如铁性凋亡,TNF信号通路,MAPK信号通路,和NF-κB信号通路被发现。几个miRNA被鉴定为hub基因的共享靶标。与健康组相比,ICH组显示单核细胞谱系和髓样树突状细胞的浸润增加。十种潜在的小分子药物(例如Zebularine,TWS-119,CG-930)通过CMap预测。
■线粒体自噬和铁凋亡之间的几个共享基因可能通过TNF驱动ICH进展,MAPK,和NF-κB途径。这些结果为进一步探索线粒体自噬之间的联系提供了有价值的见解,铁性凋亡,和ICH。
UNASSIGNED: Mitophagy and ferroptosis occur in intracerebral haemorrhage (ICH) but our understanding of mitophagy and ferroptosis-related genes remains incomplete.
UNASSIGNED: This study aims to identify shared ICH genes for both processes.
UNASSIGNED: ICH differentially expressed mitophagy and ferroptosis-related genes (DEMFRGs) were sourced from the GEO database and literature. Enrichment analysis elucidated functions. Hub genes were selected via STRING, MCODE, and MCC algorithms in Cytoscape. miRNAs targeting hubs were predicted using miRWalk 3.0, forming a miRNA-hub gene network. Immune microenvironment variances were assessed with MCP and TIMER. Potential small molecules for ICH were forecasted via CMap database.
UNASSIGNED: 64 DEMFRGs and ten hub genes potentially involved in various processes like ferroptosis, TNF signalling pathway, MAPK signalling pathway, and NF-kappa B signalling pathway were discovered. Several miRNAs were identified as shared targets of hub genes. The ICH group showed increased infiltration of monocytic lineage and myeloid dendritic cells compared to the Healthy group. Ten potential small molecule drugs (e.g. Zebularine, TWS-119, CG-930) were predicted via CMap.
UNASSIGNED: Several shared genes between mitophagy and ferroptosis potentially drive ICH progression via TNF, MAPK, and NF-kappa B pathways. These results offer valuable insights for further exploring the connection between mitophagy, ferroptosis, and ICH.