小肠细菌过度生长(SIBO)通常发生,很难治疗,并且经常复发。牛初乳(BC)和鸡蛋含有免疫球蛋白和其他具有抗菌作用的成分,免疫调节,和生长因子活动;然而,尚不清楚它们是否有能力减少与SIBO(链球菌,大肠杆菌,葡萄球菌,拟杆菌,克雷伯菌属,肠球菌,和变形杆菌)和感染性腹泻(肠致病性大肠杆菌,沙门氏菌)。我们检查了BC的影响,鸡蛋,或组合,关于细菌生长和细菌诱导的跨上皮电阻(TEER)变化以及跨融合的Caco-2单层的细菌易位。BC,鸡蛋,或组合不影响细菌生长。在单层中添加细菌会降低TEER,并且(物种之间的差异很小)增加细菌易位,单层细胞凋亡增加(caspase-3和Baxα增加,减少Bcl2),增加的细胞间粘附分子1(ICAM-1),和减少的细胞粘附分子zonulin1(ZO1)和claudin-1。BC,鸡蛋,或组合降低了这些作用(所有p<0.01)并引起血管内皮生长因子(VEGF)和热休克蛋白70(Hsp70)表达的额外增加。我们得出的结论是,BC±egg可以增强粘膜的完整性,以抵抗与SIBO和感染性腹泻相关的一系列细菌。口服BC±卵可能对这些疾病有临床价值,尤其是SIBO,其中沉淀生物的根除可能很难实现。
Small intestinal bacterial overgrowth (SIBO) occurs commonly, is difficult to treat, and frequently recurs. Bovine colostrum (BC) and chicken eggs contain immunoglobulins and other components that possess antimicrobial, immunoregulatory, and growth factor activities; however, it is not known if they have the ability to reduce injury caused by the presence of bacteria associated with SIBO (Streptococcus, Escherichia coli, Staphylococcus, Bacteroides, Klebsiella, Enterococcus, and Proteus) and infectious diarrhea (enteropathogenic Escherichia coli, Salmonella). We examined the effects of BC, egg, or the combination, on bacterial growth and bacteria-induced changes in transepithelial electrical resistance (TEER) and bacterial translocation across confluent Caco-2 monolayers. BC, egg, or the combination did not affect bacterial growth. Adding bacteria to monolayers reduced TEER and (with minor variations among species) increased bacterial translocation, increased monolayer apoptosis (increased caspase-3 and Baxα, reduced Bcl2), increased intercellular adhesion molecule 1 (ICAM-1), and reduced cell adhesion molecules zonulin1 (ZO1) and claudin-1. BC, egg, or the combination reduced these effects (all p < 0.01) and caused additional increases in vascular endothelial growth factor (VEGF) and Heat Shock Protein 70 (Hsp70) expression. We conclude that BC ± egg strengthens mucosal integrity against a battery of bacteria relevant for SIBO and for infectious diarrhea. Oral BC ± egg may have clinical value for these conditions, especially SIBO where eradication of precipitating organisms may be difficult to achieve.