背景:面部皮肤发红是一个重要的美容问题。尽管皮肤表面皮脂的定性和定量修饰是慢性炎症性皮肤病的主要致病因素,皮肤发红的关系,皮脂,健康受试者脸颊上的轻度炎症仍然难以捉摸。
目的:我们旨在探讨健康受试者脸颊发红与角质层(SC)中皮脂和炎性细胞因子之间的相关性。我们还检查了代表性皮脂脂质对培养的角质形成细胞中炎性细胞因子基因表达的影响。
方法:这项研究包括198名健康参与者。使用流动注射分析法分析皮肤皮脂,使用分光光度计评估皮肤发红。使用酶联免疫吸附测定法测量胶带剥离的SC中的炎性细胞因子。
结果:脸颊发红参数与皮肤皮脂的量和皮脂中单不饱和脂肪酸(C16:1和C18:1)的比例呈正相关。它们还与SC中的白介素(IL)-36γ/IL-37比率呈正相关。在检查的代表性皮脂中,油酸(C18:1,顺式9)剂量和时间依赖性地调节培养的角质形成细胞中IL-36γ和IL-37的mRNA表达,N-甲基-D-天冬氨酸(NMDA)型谷氨酸受体拮抗剂减弱了这种作用,MK801.
结论:健康受试者的皮肤表面皮脂可能与脸颊发红有关,和油酸通过NMDA型谷氨酸受体诱导的IL-36γ可能是它们之间的联系。我们的研究提供了一种可能的护肤策略,通过针对面部皮肤皮脂来减轻皮肤发红的不利增加,特别是油酸。
BACKGROUND: Redness of the facial skin is an important cosmetic concern. Although qualitative and quantitative modifications of sebum on the skin surface are major pathogenic factors of chronic inflammatory skin conditions, the relationship between skin redness, sebum, and mild inflammation on the cheeks of healthy subjects remains elusive.
OBJECTIVE: We aimed to explore the correlation between cheek redness and sebum and inflammatory cytokines in the stratum corneum (SC) of healthy subjects. We also examined the effects of representative sebum lipids on the gene expression of inflammatory cytokines in cultured keratinocytes.
METHODS: This study included 198 healthy participants. Skin sebum was analyzed using flow injection analysis, and skin redness was assessed using a spectrophotometer. Inflammatory cytokines in tape-stripped SC were measured using enzyme-linked immunosorbent assay.
RESULTS: Cheek redness parameters positively correlated with the amount of skin sebum and the proportion of monounsaturated free fatty acids (C16:1 and C18:1) in the sebum. They also positively correlated with the interleukin (IL)-36γ/IL-37 ratio in the SC. Among the representative sebum lipids examined, oleic acid (C18:1, cis-9) dose- and time-dependently regulated the mRNA expression of IL-36γ and IL-37 in cultured keratinocytes, and this effect was attenuated by the N-methyl-D-aspartate (NMDA)-type glutamate receptor antagonist, MK801.
CONCLUSIONS: Skin surface sebum may be related to cheek redness in healthy subjects, and oleic acid-induced IL-36γ through NMDA-type glutamate receptors may be a link between them. Our study provides a possible skincare strategy for mitigating unfavorable increase in skin redness by targeting the facial skin sebum, particularly oleic acid.